pEMS1062 | Addgene 29011 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEMS1062

catalog :

29011

citations: 3

Reference
Korecki A, Cueva Vargas J, Fornes O, Agostinone J, Farkas R, Hickmott J, et al. Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, corneal, endothelial, Müller glial, and PAX6 cells. Gene Ther. 2021;28:351-372 pubmed publisher
de Leeuw C, Dyka F, Boye S, Laprise S, Zhou M, Chou A, et al. Targeted CNS Delivery Using Human MiniPromoters and Demonstrated Compatibility with Adeno-Associated Viral Vectors. Mol Ther Methods Clin Dev. 2014;1:5 pubmed
Portales Casamar E, Swanson D, Liu L, de Leeuw C, Banks K, Ho Sui S, et al. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010;107:16589-94 pubmed publisher

product information

Catalog Number :

29011

Product Name :

pEMS1062

article :

doi	10.1038/s41434-021-00227-z
id	28211774
pubmed_id	33531684

bacterial resistance :

Ampicillin

cloning :

backbone

pEMS1040

backbone_mutation

backbone_origin

backbone_size

17590

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

High copy number homologous recombination plasmid for gene targeting at the mouse Hprt locus

growth notes :

Additional References: 1 Korecki, A. J. et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics 211, 1155-1177 (2019). 2 de Leeuw, C. N. et al. rAAV-Compatible MiniPromoters for Restricted Expression in the Brain and Eye. Molecular Brain 9, 52 (2016). 3 Scalabrino, M. L. et al. Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol Genet 24, 6229-6239, doi:10.1093/hmg/ddv341, ddv341 [pii] (2015). 4 de Leeuw, C. N. et al. Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Mol Ther Methods Clin Dev 1, 1-15 (2014).

growth strain :

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

growth temp :

DH10B

origin :

pi :

alt_names

PCP2

Purkinje cell protein 2

cloning

clone_method	Restriction Enzyme
cloning_site_3	MluI
cloning_site_5	SacI
promoter	MiniPromoter Ple155 from the human PCP2 gene
sequencing_primer_3
sequencing_primer_5	Unknown
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

Ple155-Cre/EGFP-NLS

shRNA_sequence

size

1652

species