This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-C1-AR
catalog :
28235
citations: 26
Reference
Basu S, Mart xed nez Crist xf3 bal P, Frigol xe9 Vivas M, Pesarrodona M, Lewis M, Szulc E, et al. Rational optimization of a transcription factor activation domain inhibitor. Nat Struct Mol Biol. 2023;30:1958-1969 pubmed publisher
Zhang S, Zeng L, Su B, Yang G, Wang J, Ming S, et al. The glycoprotein 5 of porcine reproductive and respiratory syndrome virus stimulates mitochondrial ROS to facilitate viral replication. MBio. 2023;14:e0265123 pubmed publisher
Kwon H, Schafer J, Song N, Kaneko S, Li A, Xiao T, et al. Androgen conspires with the CD8+ T cell exhaustion program and contributes to sex bias in cancer. Sci Immunol. 2022;7:eabq2630 pubmed publisher
Mao F, Kong Y, Liu J, Rao X, Li C, Donahue K, et al. Diptoindonesin G antagonizes AR signaling and enhances the efficacy of antiandrogen therapy in prostate cancer. Prostate. 2022;82:917-932 pubmed publisher
Zhang N, Bewick B, Schultz J, Tiwari A, Krencik R, Zhang A, et al. DNAzyme Cleavage of CAG Repeat RNA in Polyglutamine Diseases. Neurotherapeutics. 2021;: pubmed publisher
Schmidt K, Weidmann C, Hilimire T, Yee E, Hatfield B, Schneekloth J, et al. Targeting the Oncogenic Long Non-coding RNA SLNCR1 by Blocking Its Sequence-Specific Binding to the Androgen Receptor. Cell Rep. 2020;30:541-554.e5 pubmed publisher
Kim K, Lee D, Park S, Jo S, Ku B, Park S, et al. Disordered region of cereblon is required for efficient degradation by proteolysis-targeting chimera. Sci Rep. 2019;9:19654 pubmed publisher
Grolez G, Gordiendko D, Clarisse M, Hammadi M, Desruelles E, Fromont G, et al. TRPM8-androgen receptor association within lipid rafts promotes prostate cancer cell migration. Cell Death Dis. 2019;10:652 pubmed publisher
Kuo P, Tseng J, Chen H, Wu C, Omar H, Wang C, et al. Identification of SEPTIN12 as a novel target of the androgen and estrogen receptors in human testicular cells. Biochimie. 2019;158:1-9 pubmed publisher
Bouchard J, Otero J, Scott D, Szulc E, Martin E, Sabri N, et al. Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments. Mol Cell. 2018;72:19-36.e8 pubmed publisher
Li D, Tian G, Wang J, Zhao L, Co O, Underill Z, et al. Inhibition of androgen receptor transactivation function by adenovirus type 12 E1A undermines prostate cancer cell survival. Prostate. 2018;78:1140-1156 pubmed publisher
Chopra H, Khan Z, Contreras J, Wang H, Sedrak A, Zhu Y. Activation of p53 and destabilization of androgen receptor by combinatorial inhibition of MDM2 and MDMX in prostate cancer cells. Oncotarget. 2018;9:6270-6281 pubmed publisher
De Mol E, Szulc E, Di Sanza C, Martínez Cristóbal P, Bertoncini C, Fenwick R, et al. Regulation of Androgen Receptor Activity by Transient Interactions of Its Transactivation Domain with General Transcription Regulators. Structure. 2018;26:145-152.e3 pubmed publisher
Kato Y, Ochiai K, Kawakami S, Nakao N, Azakami D, Bonkobara M, et al. Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines. BMC Vet Res. 2017;13:170 pubmed publisher
Sreenath T, Macalindong S, Mikhalkevich N, Sharad S, Mohamed A, Young D, et al. ETS Related Gene mediated Androgen Receptor Aggregation and Endoplasmic Reticulum Stress in Prostate Cancer Development. Sci Rep. 2017;7:1109 pubmed publisher
Chattopadhyay I, Wang J, Qin M, Gao L, Holtz R, Vessella R, et al. Src promotes castration-recurrent prostate cancer through androgen receptor-dependent canonical and non-canonical transcriptional signatures. Oncotarget. 2017;8:10324-10347 pubmed publisher
Read J, Ingram A, Al Saleh H, Platko K, Gabriel K, Kapoor A, et al. Nuclear transportation of exogenous epidermal growth factor receptor and androgen receptor via extracellular vesicles. Eur J Cancer. 2017;70:62-74 pubmed publisher
Corso Díaz X, de Leeuw C, Alonso V, Melchers D, Wong B, Houtman R, et al. Co-activator candidate interactions for orphan nuclear receptor NR2E1. BMC Genomics. 2016;17:832 pubmed
Yamaguchi A, Takanashi K. FUS interacts with nuclear matrix-associated protein SAFB1 as well as Matrin3 to regulate splicing and ligand-mediated transcription. Sci Rep. 2016;6:35195 pubmed publisher
Patsch K, Chiu C, Engeln M, Agus D, Mallick P, Mumenthaler S, et al. Single cell dynamic phenotyping. Sci Rep. 2016;6:34785 pubmed publisher
Schmidt K, Joyce C, Buquicchio F, Brown A, Ritz J, Distel R, et al. The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like Region. Cell Rep. 2016;15:2025-37 pubmed publisher
Chiu C, Patsch K, Cutrale F, Soundararajan A, Agus D, Fraser S, et al. Intracellular kinetics of the androgen receptor shown by multimodal Image Correlation Spectroscopy (mICS). Sci Rep. 2016;6:22435 pubmed publisher
Ochiai K, Morimatsu M, Kato Y, Ishiguro Oonuma T, Udagawa C, Rungsuriyawiboon O, et al. Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity. Oncotarget. 2016;7:3283-96 pubmed publisher
Doberstein K, Milde Langosch K, Bretz N, Schirmer U, Harari A, Witzel I, et al. L1CAM is expressed in triple-negative breast cancers and is inversely correlated with androgen receptor. BMC Cancer. 2014;14:958 pubmed publisher
Golovine K, Makhov P, Teper E, Kutikov A, Canter D, Uzzo R, et al. Piperlongumine induces rapid depletion of the androgen receptor in human prostate cancer cells. Prostate. 2013;73:23-30 pubmed publisher
Stenoien D, Cummings C, Adams H, Mancini M, Patel K, DeMartino G, et al. Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. Hum Mol Genet. 1999;8:731-41 pubmed
product information
Catalog Number :
28235
Product Name :
pEGFP-C1-AR
article :
doi10.1093/hmg/8.5.731
id4075
pubmed_id10196362
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addgene's sequencing result indicates a loss of Q58 in the unstable poly-glutamine region. Our sequencing also shows that the BamHI site at the 3' end of the insert was destroyed. Please see our quality control sequence for the most accurate look at this region. Tilley, W.D., Marcelli, M., Wilson, J.D. and McPhaul, J.M. (1989) Characterization and cloning of a cDNA encoding the human androgen receptor. Proc. Natl Acad. Sci. USA, 86, 327 331
growth strain :
Mammalian expression of Androgen receptor fused to EGFP
growth temp :
DH5 alpha, XL10 gold
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5Kpn I
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesAIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
geneAR
id367
genbank_ids
NM_000044
mutation
nameAndrogen receptor
shRNA_sequence
size2760
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
plasmid copy :
pCMV-AR
resistance markers :
922
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA