This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SI1-Unc5b
catalog :
28214
citations: 1
Reference
Crosnier C, Staudt N, Wright G. A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. BMC Biol. 2010;8:76 pubmed publisher
product information
Catalog Number :
28214
Product Name :
SI1-Unc5b
article :
doi10.1186/1741-7007-8-76
id4070
pubmed_id20525357
bacterial resistance :
Ampicillin
cloning :
backbonepTT3
backbone_mutation
backbone_originYves Durocher, NRC
backbone_size5925
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See map and Genbank file for plasmid details. Please note that Addgene's sequencing results identified 4 single nucleotide mismatches (at bp# 3373, 3376, 3380 and 3386) when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.
origin :
37
pi :
alt_names
Unc5b
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5M13_puc_Rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesunc5h2
geneunc5b
id494130
genbank_ids
mutation
nameUnc5b mouse monoclonal antibody
shRNA_sequence
size
species
7955
Danio rerio
tags
plasmid copy :
pTT3 was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute
resistance markers :
919
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA