pEB-C5 | Addgene 28213 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEB-C5

catalog :

28213

citations: 8

Reference
Scalzo S, Afonso M, da Fonseca N, Jesus I, Alves A, Mendon xe7 a C, et al. Dense optical flow software to quantify cellular contractility. Cell Rep Methods. 2021;1:100044 pubmed publisher
Borsoi J, Morato Marques M, de Araújo Tofoli F, Assis Pereira L, Farinha Arcieri L, Delgado Sarafian R, et al. Generation of two human induced pluripotent stem cell (hiPSC) lines derived from unrelated Marfan Syndrome patients. Stem Cell Res. 2021;54:102407 pubmed publisher
Vil xe0 Gonz xe1 lez M, Eleftheriadou M, Kelaini S, Naderi Meshkin H, Flanagan S, Stewart S, et al. Endothelial Cells Derived From Patients With Diabetic Macular Edema Recapitulate Clinical Evaluations of Anti-VEGF Responsiveness Through the Neuronal Pentraxin 2 Pathway. Diabetes. 2020;69:2170-2185 pubmed publisher
Khoshchehreh R, Totonchi M, Carlos Ramírez J, Torres R, Baharvand H, Aicher A, et al. Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity. Oncogene. 2019;38:6226-6239 pubmed publisher
Junqueira Reis L, Pican xe7 o Castro V, Paes B, Pereira O, Gerdes Gyuricza I, de Ara xfa jo F, et al. Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia. Stem Cells Int. 2017;2017:7492914 pubmed publisher
Kim S, Oceguera Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, et al. KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015;4:727-43 pubmed publisher
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458-66 pubmed publisher
Chou B, Mali P, Huang X, Ye Z, Dowey S, Resar L, et al. Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011;21:518-29 pubmed publisher

product information

Catalog Number :

28213

Product Name :

pEB-C5

article :

doi	10.1038/cr.2011.12
id	4069
pubmed_id	21243013

bacterial resistance :

Ampicillin

cloning :

backbone

pCEP4

backbone_mutation

backbone_origin

invitrogen

backbone_size

10200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note that Addgene's sequencing results using the CMV-F and pCEP-F primers found 2 sequence variants within and after the CMV enhancer. One is a C->G mismatch at position 775 within the CMV enhancer and the second is a deletion at position 949 just after the end of the CAG enhancer, as compared to the full plasmid sequence provided by the depositing scientist. The significance of the two sequence variants is unclear; however, the plasmid functions as described in the associated publication. Addgene's other 4 sequencing results completely matched the depositor's sequence.

growth strain :

5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

growth temp :

STBL3

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	N/A
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Myc2, Niard, Nird, bHLHe39
gene	Myc
id	17869

aliases	Sox-2, lcc, ysb
gene	Sox2
id	20674

aliases	NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
gene	Pou5f1
id	18999

aliases	EZF, Gklf, Zie
gene	Klf4
id	16600

aliases	Gm10299, Lin-28, Lin28, Tex17, lin-28A
gene	Lin28a
id	83557

genbank_ids

mutation

name

Oct4, Sox2, Klf4, c-Myc, Lin28

shRNA_sequence

size

7612

species

10090

Mus musculus