This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
E-cadherin-GFP
catalog :
28009
citations: 30
Reference
Nzigou Mombo B, Bijonowski B, Raab C, Niland S, Brockhaus K, M xfc ller M, et al. Reversible photoregulation of cell-cell adhesions with opto-E-cadherin. Nat Commun. 2023;14:6292 pubmed publisher
Tapadar P, Pal A, Ghosal N, Kumar B, Paul T, Biswas N, et al. CDH1 overexpression sensitizes TRAIL resistant breast cancer cells towards rhTRAIL induced apoptosis. Mol Biol Rep. 2023;: pubmed publisher
Alvizi L, Nani D, Brito L, Kobayashi G, Passos Bueno M, Mayor R. Neural crest E-cadherin loss drives cleft lip/palate by epigenetic modulation via pro-inflammatory gene-environment interaction. Nat Commun. 2023;14:2868 pubmed publisher
Baro L, Islam A, Brown H, Bell Z, Juanes M. APC-driven actin nucleation powers collective cell dynamics in colorectal cancer cells. iScience. 2023;26:106583 pubmed publisher
Han W, He M, Zhang Y, Zhou J, Li Z, Liu X, et al. Cadherin-dependent adhesion modulated 3D cell-assembly. J Mater Chem B. 2022;10:4959-4966 pubmed publisher
Begum H, Mariano C, Zhou H, Shen K. E-Cadherin Regulates Mitochondrial Membrane Potential in Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
Damiano V, Spessotto P, Vanin G, Perin T, Maestro R, Santarosa M. The Autophagy Machinery Contributes to E-cadherin Turnover in Breast Cancer. Front Cell Dev Biol. 2020;8:545 pubmed publisher
Munthe E, Raiborg C, Stenmark H, Wenzel E. Clathrin regulates Wnt/β-catenin signaling by affecting Golgi to plasma membrane transport of transmembrane proteins. J Cell Sci. 2020;133: pubmed publisher
Bartle E, Rao T, Beggs R, Dean W, Urner T, Kowalczyk A, et al. Protein exchange is reduced in calcium-independent epithelial junctions. J Cell Biol. 2020;219: pubmed publisher
Ollech D, Pflästerer T, Shellard A, Zambarda C, Spatz J, Marcq P, et al. An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells. Nat Commun. 2020;11:472 pubmed publisher
Jia D, Augert A, Kim D, Eastwood E, Wu N, Ibrahim A, et al. Crebbp Loss Drives Small Cell Lung Cancer and Increases Sensitivity to HDAC Inhibition. Cancer Discov. 2018;8:1422-1437 pubmed publisher
Wieduwild R, Howarth M. Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions. Biomaterials. 2018;180:253-264 pubmed publisher
Liu M, Jiang K, Lin G, Liu P, Yan Y, Ye T, et al. Ajuba inhibits hepatocellular carcinoma cell growth via targeting of β-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation. J Exp Clin Cancer Res. 2018;37:165 pubmed publisher
Zahedi A, Phandthong R, Chaili A, Remark G, Talbot P. Epithelial-to-mesenchymal transition of A549 lung cancer cells exposed to electronic cigarettes. Lung Cancer. 2018;122:224-233 pubmed publisher
Sehgal P, Kong X, Wu J, Sunyer R, Trepat X, Leckband D. Epidermal growth factor receptor and integrins control force-dependent vinculin recruitment to E-cadherin junctions. J Cell Sci. 2018;131: pubmed publisher
Kievit A, Tessadori F, Douben H, Jordens I, Maurice M, Hoogeboom J, et al. Variants in members of the cadherin-catenin complex, CDH1 and CTNND1, cause blepharocheilodontic syndrome. Eur J Hum Genet. 2018;26:210-219 pubmed publisher
Piotrowski Daspit A, Nerger B, Wolf A, Sundaresan S, Nelson C. Dynamics of Tissue-Induced Alignment of Fibrous Extracellular Matrix. Biophys J. 2017;113:702-713 pubmed publisher
Merk H, Messer P, Ardelt M, Lamb D, Zahler S, Muller R, et al. Inhibition of the V-ATPase by Archazolid A: A New Strategy to Inhibit EMT. Mol Cancer Ther. 2017;16:2329-2339 pubmed publisher
Burda P, Caldelari R, Heussler V. Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress. MBio. 2017;8: pubmed publisher
Ghoumid J, Stichelbout M, Jourdain A, Frénois F, Lejeune Dumoulin S, Alex Cordier M, et al. Blepharocheilodontic syndrome is a CDH1 pathway-related disorder due to mutations in CDH1 and CTNND1. Genet Med. 2017;19:1013-1021 pubmed publisher
Hu B, Arpag S, Zhang X, Mobius W, Werner H, SOSINSKY G, et al. Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP. PLoS Genet. 2016;12:e1006290 pubmed publisher
Cai Z, Zhang A, Choksi S, Li W, Li T, Zhang X, et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res. 2016;26:886-900 pubmed publisher
Martínez Martinez M, de Juan Romero C, Fernandez V, Cárdenas A, Gotz M, Borrell V. A restricted period for formation of outer subventricular zone defined by Cdh1 and Trnp1 levels. Nat Commun. 2016;7:11812 pubmed publisher
Sroka R, Van Lint J, Katz S, Schneider M, Kleger A, Paschke S, et al. Cortactin is a scaffolding platform for the E-cadherin adhesion complex and is regulated by protein kinase D1 phosphorylation. J Cell Sci. 2016;129:2416-29 pubmed publisher
Milano D, Ngai N, Muthuswamy S, Asthagiri A. Regulators of Metastasis Modulate the Migratory Response to Cell Contact under Spatial Confinement. Biophys J. 2016;110:1886-1895 pubmed publisher
Shashikanth N, Kisting M, Leckband D. Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions. Sci Rep. 2016;6:23623 pubmed publisher
Fiorino C, Harrison R. E-cadherin is important for cell differentiation during osteoclastogenesis. Bone. 2016;86:106-18 pubmed publisher
Piotrowski Daspit A, Tien J, Nelson C. Interstitial fluid pressure regulates collective invasion in engineered human breast tumors via Snail, vimentin, and E-cadherin. Integr Biol (Camb). 2016;8:319-31 pubmed publisher
Yin H, Copley C, Goodrich L, Deans M. Comparison of phenotypes between different vangl2 mutants demonstrates dominant effects of the Looptail mutation during hair cell development. PLoS ONE. 2012;7:e31988 pubmed publisher
Miranda K, Khromykh T, Christy P, Le T, Gottardi C, Yap A, et al. A dileucine motif targets E-cadherin to the basolateral cell surface in Madin-Darby canine kidney and LLC-PK1 epithelial cells. J Biol Chem. 2001;276:22565-72 pubmed
product information
Catalog Number :
28009
Product Name :
E-cadherin-GFP
article :
doi10.1074/jbc.M101907200
id4019
pubmed_id11312273
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note the EGFP protein sequence in this plasmid is missing the first two amino acids of the standard EGFP sequence (Met-Val) due to the cloning strategy. There is also a Y238I mutation present at the C-terminus of EGFP. These differences do not affect the fluorescent protein properties as described in the associated publication.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5Unknown
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesArc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO
geneCDH1
id999
genbank_ids
mutation
nameE-cadherin
shRNA_sequence
size2648
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
plasmid copy :
Human Ecadherin gene in pCDNA3.1 we had from Alpha Yap laboratory (University of Queensland in 1998) We inserted GFP at C- terminal of Human Ecadherin in pcDNA3.1
resistance markers :
907
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA