This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CAV1-P132L-mEGFP
catalog :
27708
citations: 4
Reference
Deng Y, Moo E, Inoue A, Br xe4 uner Osborne H. Endocytic proteins mediating GPR15 receptor internalization provide insight into the underlying mechanisms. FEBS Lett. 2023;597:1528-1540 pubmed publisher
Kj xe6 r V, Daugvilaite V, Stępniewski T, Madsen C, J xf8 rgensen A, Bhuskute K, et al. Migration mediated by the oxysterol receptor GPR183 depends on arrestin coupling but not receptor internalization. Sci Signal. 2023;16:eabl4283 pubmed publisher
Azizi P, Zyla R, Guan S, Wang C, Liu J, Bolz S, et al. Clathrin-dependent entry and vesicle-mediated exocytosis define insulin transcytosis across microvascular endothelial cells. Mol Biol Cell. 2015;26:740-50 pubmed publisher
Hayer A, Stoeber M, Bissig C, Helenius A. Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Traffic. 2010;11:361-82 pubmed publisher
product information
Catalog Number :
27708
Product Name :
CAV1-P132L-mEGFP
article :
doi10.1111/j.1600-0854.2009.01023.x
id3998
pubmed_id20070607
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size3961
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The CAV1 mutant P132L accumulates in ER/Golgi, has oligomerisation defects, and does not support caveolae formation.
origin :
37
pi :
alt_names
CAV1, cav-1
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3ACC TCT ACA AAT GTG GTA TGG CTG
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasescaveolin 1
geneCAV1
id403980
genbank_ids
NM_001003296
mutationP132L
namecaveolin-1
shRNA_sequence
size1322
species
Canis lupus familiaris
tags
locationC terminal on insert
tagmonomeric EGFP
resistance markers :
198
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA