CALM-pmCherryN1 | Addgene 27691 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CALM-pmCherryN1

catalog :

27691

citations: 4

Reference
Catsburg L, Westra M, van Schaik A, MacGillavry H. Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses. elife. 2022;11: pubmed publisher
Scott B, Sochacki K, Low Nam S, Bailey E, Luu Q, Hor A, et al. Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics. Nat Commun. 2018;9:419 pubmed publisher
Garcia Alai M, Heidemann J, Skruzny M, Gieras A, Mertens H, Svergun D, et al. Epsin and Sla2 form assemblies through phospholipid interfaces. Nat Commun. 2018;9:328 pubmed publisher
Taylor M, Perrais D, Merrifield C. A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis. PLoS Biol. 2011;9:e1000604 pubmed publisher

product information

Catalog Number :

27691

Product Name :

CALM-pmCherryN1

article :

doi	10.1371/journal.pbio.1000604
id	4268
pubmed_id	21445324

bacterial resistance :

Kanamycin

cloning :

backbone

pmCherry-N1

backbone_mutation

backbone_origin

Clontech

backbone_size

4722

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Cloned from IMAGE clone: 2651109 (MGC:19382). This plasmid may be slow growing in bacteria. For difficult constructs you can use TG1s (they are Endonuclease positive, but good for cloning) and INV F' E.coli strains. The latter give the best results. Also when growing in culture try to switch to 2xTY media, which is richer that LB.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XmaI
cloning_site_5	XhoI
promoter
sequencing_primer_3	mCherry-R 5'-TTGGTCACCTTCAGCTTGG
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CALM, CLTH, PAP180, fit-1, fit1, mKIAA4114
gene	Picalm
id	233489

genbank_ids

mutation

name

CALM

shRNA_sequence

size

2000

species

10090

Mus musculus