This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AP2u2-mCherry
catalog :
27672
citations: 6
Reference
Hellen N, Mashanov G, Conte I, Le Trionnaire S, Babich V, Knipe L, et al. P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane. Nat Commun. 2022;13:3031 pubmed publisher
Işbilir A, Möller J, Arimont M, Bobkov V, Perpina Viciano C, Hoffmann C, et al. Advanced fluorescence microscopy reveals disruption of dynamic CXCR4 dimerization by subpocket-specific inverse agonists. Proc Natl Acad Sci U S A. 2020;117:29144-29154 pubmed publisher
Cai X, Wang H, Wang M, Wang D, Zhang Z, Wei R, et al. A novel phosphorylation site on orexin receptor 1 regulating orexinA-induced GRK2-biased signaling. Cell Signal. 2020;75:109743 pubmed publisher
Yu C, Rafiq N, Cao F, Zhou Y, Krishnasamy A, Biswas K, et al. Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force. Nat Commun. 2015;6:8672 pubmed publisher
Lampe M, Pierre F, Al Sabah S, Krasel C, Merrifield C. Dual single-scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered ?2-adrenergic receptor (?2AR) or Mu-opioid receptor (MOR) endocytosis. Mol Biol Cell. 2014;25:3070-80 pubmed publisher
Taylor M, Perrais D, Merrifield C. A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis. PLoS Biol. 2011;9:e1000604 pubmed publisher
product information
Catalog Number :
27672
Product Name :
AP2u2-mCherry
article :
doi10.1371/journal.pbio.1000604
id4268
pubmed_id21445324
bacterial resistance :
Ampicillin
cloning :
backbonepIRESneo3
backbone_mutation
backbone_originClontech
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Rat 2 was cloned from a construct provided by David Owen (see Collins et al. 2002). mCherry was inserted within 2 at residue 236 with BglII and NdeI sites.
origin :
37
pi :
alt_names
mu2
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5NheI
promoter
sequencing_primer_3IRES-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAp50, Clapm1, mu2
geneAp2m1
id116563
genbank_ids
mutation
nameAp2m1
shRNA_sequence
size1400
species
10116
Rattus norvegicus
tags
location
tagmCherry
resistance markers :
900
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA