This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABEpuro U3-hTR-500
catalog :
27666
citations: 6
Reference
Takasugi T, Gu P, Liang F, Staco I, Chang S. Pot1b -/- tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation. Nucleic Acids Res. 2023;51:9227-9247 pubmed publisher
Gu P, Jia S, Takasugi T, Tesmer V, Nandakumar J, Chen Y, et al. Distinct functions of POT1 proteins contribute to the regulation of telomerase recruitment to telomeres. Nat Commun. 2021;12:5514 pubmed publisher
Dagg R, Pickett H, Neumann A, Napier C, Henson J, Teber E, et al. Extensive Proliferation of Human Cancer Cells with Ever-Shorter Telomeres. Cell Rep. 2017;19:2544-2556 pubmed publisher
Min J, Wright W, Shay J. Alternative lengthening of telomeres can be maintained by preferential elongation of lagging strands. Nucleic Acids Res. 2017;45:2615-2628 pubmed publisher
Majumder M, House R, Palanisamy N, Qie S, Day T, Neskey D, et al. RNA-Binding Protein FXR1 Regulates p21 and TERC RNA to Bypass p53-Mediated Cellular Senescence in OSCC. PLoS Genet. 2016;12:e1006306 pubmed publisher
Wong J, Collins K. Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita. Genes Dev. 2006;20:2848-58 pubmed
product information
Catalog Number :
27666
Product Name :
pBABEpuro U3-hTR-500
article :
doi10.1101/gad.1476206
id3994
pubmed_id17015423
bacterial resistance :
Ampicillin
cloning :
backbonepBABEpuro
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
sequence is for the insert of human U3 snoRNA promoter driving expression of human telomerase RNA mature sequence (hTR) with 500 bp downstream genomic region included; see Fu and Collins (2003)
origin :
37
pi :
alt_names
human telomerase RNA
TER
TERC
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5NheI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDKCA1, PFBMFT2, SCARNA19, TER, TR, TRC3, hTR
geneTERC
id7012
genbank_ids
mutation
nameU3-hTR-500
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
resistance markers :
892
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA