This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABEpuroUThTERT+U3-hTR-500
catalog :
27665
citations: 4
Reference
Mangosh T, Awadallah W, Grabowska M, Taylor D. SLX4IP Promotes Telomere Maintenance in Androgen Receptor-Independent Castration-Resistant Prostate Cancer through ALT-like Telomeric PML Localization. Mol Cancer Res. 2021;19:301-316 pubmed publisher
Zeng X, Hernandez Sanchez W, Xu M, Whited T, Baus D, Zhang J, et al. Administration of a Nucleoside Analog Promotes Cancer Cell Death in a Telomerase-Dependent Manner. Cell Rep. 2018;23:3031-3041 pubmed publisher
Dagg R, Pickett H, Neumann A, Napier C, Henson J, Teber E, et al. Extensive Proliferation of Human Cancer Cells with Ever-Shorter Telomeres. Cell Rep. 2017;19:2544-2556 pubmed publisher
Wong J, Collins K. Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita. Genes Dev. 2006;20:2848-58 pubmed
product information
Catalog Number :
27665
Product Name :
pBABEpuroUThTERT+U3-hTR-500
article :
doi10.1101/gad.1476206
id3994
pubmed_id17015423
bacterial resistance :
Ampicillin
cloning :
backbonepBABEpuro
backbone_mutation
backbone_origin
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
untagged (UT) human TERT (hTERT) into pBABEpuro U3-hTR-500 (U3 promoter + hTR sequence given as insert; second insert of hTERT sequence available from GenBank); this plasmid is for coexpression of hTERT and hTR
origin :
37
pi :
alt_names
human telomerase RNA
TER
TERC
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SnaB1
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDKCA1, PFBMFT2, SCARNA19, TER, TR, TRC3, hTR
geneTERC
id7012
aliasesCMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT
geneTERT
id7015
genbank_ids
mutation
nameUThTERT+U3-hTR-500
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
resistance markers :
892
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA