pCMV4-NIK-HA | Addgene 27554 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV4-NIK-HA

catalog :

27554

citations: 4

Reference
Kamradt M, Jung J, Pflug K, Lee D, Fanniel V, Sitcheran R. NIK promotes metabolic adaptation of glioblastoma cells to bioenergetic stress. Cell Death Dis. 2021;12:271 pubmed publisher
D Ignazio L, Batie M, Rocha S. TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway. Cells. 2018;7: pubmed publisher
Shi M, Cho H, Inn K, Yang A, Zhao Z, Liang Q, et al. Negative regulation of NF-ÎºB activity by brain-specific TRIpartite Motif protein 9. Nat Commun. 2014;5:4820 pubmed publisher
Xiao G, Sun S. Negative regulation of the nuclear factor kappa B-inducing kinase by a cis-acting domain. J Biol Chem. 2000;275:21081-5 pubmed

product information

Catalog Number :

27554

Product Name :

pCMV4-NIK-HA

article :

doi	10.1074/jbc.M002552200
id	3978
pubmed_id	10887201

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV4

backbone_mutation

backbone_origin

Dr. David Russell, UTSW

backbone_size

4874

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

According to the depositing laboratory, the differences in NIK compared to GenBank reference sequence NP_003945.2 do not affect the function of NIK.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRV
cloning_site_5	KpnI
promoter	CMV
sequencing_primer_3	hGH-pA-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FTDCR1B, HS, HSNIK, NIK
gene	MAP3K14
id	9020

genbank_ids

mutation

NIK starts at amino acid 3; C52R and R139H mutations when compared to NP_003945.2

name

NIK

shRNA_sequence

size

species

9606

Homo sapiens