This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pETZZtevPP7CP(C68A,C73A)
catalog :
27548
citations: 2
Reference
Meredith E, Balas M, Sindy K, Haislop K, JOHNSON A. An RNA matchmaker protein regulates the activity of the long noncoding RNA HOTAIR. RNA. 2016;22:995-1010 pubmed publisher
Hogg J, Collins K. RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation. RNA. 2007;13:868-80 pubmed
product information
Catalog Number :
27548
Product Name :
pETZZtevPP7CP(C68A,C73A)
article :
doi10.1261/rna.565207
id3976
pubmed_id17456562
bacterial resistance :
Kanamycin
cloning :
backbonepET28
backbone_mutation
backbone_origin
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
PP7CP
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoter
sequencing_primer_3
sequencing_primer_5T7 Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPP7p2
genecp
id1261104
genbank_ids
CAA56473
mutationchanged two cysteine to alanine (C68A,C73A) to inhibit coat protein polymerization into capsid
namePseudomonas phage PP7 coat protein
shRNA_sequence
size900
species
Pseudomonas phage PP7
tags
locationN terminal on insert
tagtandem protein A (ZZ)
locationN terminal on insert
tagTEV protease cleavage site
locationC terminal on backbone
tagsix histidine tag
plasmid copy :
David Peabody
resistance markers :
892
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA