pKDT17 | Addgene 27503 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pKDT17

catalog :

27503

citations: 4

Reference
Thees A, Pietrosimone K, Melchiorre C, Marden J, Graf J, Lynes M, et al. PmtA Regulates Pyocyanin Expression and Biofilm Formation in Pseudomonas aeruginosa. Front Microbiol. 2021;12:789765 pubmed publisher
Vetrivel A, Natchimuthu S, Subramanian V, Murugesan R. High-Throughput Virtual Screening for a New Class of Antagonist Targeting LasR of Pseudomonas aeruginosa. ACS Omega. 2021;6:18314-18324 pubmed publisher
Kafle P, Amoh A, Reaves J, Suneby E, Tutunjian K, Tyson R, et al. Molecular Insights into the Impact of Oxidative Stress on the Quorum-Sensing Regulator Protein LasR. J Biol Chem. 2016;291:11776-86 pubmed publisher
Pearson J, Gray K, Passador L, Tucker K, Eberhard A, Iglewski B, et al. Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proc Natl Acad Sci U S A. 1994;91:197-201 pubmed

product information

Catalog Number :

27503

Product Name :

pKDT17

article :

doi	10.1073/pnas.91.1.197
id	3967
pubmed_id	8278364

bacterial resistance :

Ampicillin

cloning :

backbone

pTS400

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Expresses LasR under the control of the lac promoter. Contains a lasB::lacZ reporter of lasB promoter activity

origin :

pi :

alt_names

transcriptional regulator LasR

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	PA1430
gene	lasR
id	881789

genbank_ids

mutation

name

LasR, LasB::LacZ fusion

shRNA_sequence

size

800

species

Pseudomonas aeruginosa