SPL10p::pCGTAG (SPL10p::NLS-GFP) | Addgene 27410 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

SPL10p::pCGTAG (SPL10p::NLS-GFP)

catalog :

27410

citations: 1

Reference
Nodine M, Bartel D. MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Genes Dev. 2010;24:2678-92 pubmed publisher

product information

Catalog Number :

27410

Product Name :

SPL10p::pCGTAG (SPL10p::NLS-GFP)

article :

doi	10.1101/gad.1986710
id	3947
pubmed_id	21123653

bacterial resistance :

Kanamycin

cloning :

backbone	pCGTAG
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

SPL10p::NLS-GFP was generated by PCR amplification of 1.9-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG. The primers used to create this construct were 5'-TCATCATCTTTCACG-3' and 5'-TCAAGATGTGGGTTG-3'. Please note that Addgene's sequencing results identified single nucleotide mismatches at bp# 11592, 11985 and 12403 when compared to the full plasmid sequence provided by the depositing laboratory. These mismatches are not known to affect the plasmid function.

growth temp :

MACH-1 strain of E. coli (from Invitrogen)

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	M13 reverse
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	F17L21.15, F17L21_15
gene	AT1G27370
id	839626

genbank_ids

mutation

SPL10 promoter transcriptionally fused to a nuclear-localized GFP

name

SPL10 promoter

shRNA_sequence

size

species

3702

Arabidopsis thaliana