UB13p::dsSPL10 v01 (SPL10-RNAi) | Addgene 27409 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

UB13p::dsSPL10 v01 (SPL10-RNAi)

catalog :

27409

citations: 1

Reference
Nodine M, Bartel D. MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Genes Dev. 2010;24:2678-92 pubmed publisher

product information

Catalog Number :

27409

Product Name :

UB13p::dsSPL10 v01 (SPL10-RNAi)

article :

doi	10.1101/gad.1986710
id	3947
pubmed_id	21123653

bacterial resistance :

Kanamycin

cloning :

backbone	UBI3pTpFSPGW-HYGv02
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

SPL10-RNAi constructs were generated by amplifying 0.6kb of the SPL10 CDS from cDNA prepared from inflorescence tissue total RNA isolated using TRIzol reagent (Invitrogen) and reverse-transcribed with oligo(dT) primers using SuperScript III Reverse Transcriptase (Invitrogen). The resulting amplicons were then cloned into pCR8/GW-TOPO (Invitrogen) and then recombined with UBI3p::pFSPGW-HYGv02 to create an inverted repeat of the SPL10 cDNA fragment under the control of the UBI3 promoter. For details regarding the UBI3p::pFSPGW-HYGv02 vector, please see the article.

growth temp :

MACH-1 strain of E. coli (from Invitrogen)

origin :

pi :

alt_names

SPL10

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13 forward 20
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	F17L21.15, F17L21_15
gene	AT1G27370
id	839626

genbank_ids

mutation

name

SPL10 inverted

shRNA_sequence

size

species

3702

Arabidopsis thaliana