6mpSPL10F13R13::pBIB-BAS (6mSPL10) | Addgene 27408 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

6mpSPL10F13R13::pBIB-BAS (6mSPL10)

catalog :

27408

citations: 1

Reference
Nodine M, Bartel D. MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Genes Dev. 2010;24:2678-92 pubmed publisher

product information

Catalog Number :

27408

Product Name :

6mpSPL10F13R13::pBIB-BAS (6mSPL10)

article :

doi	10.1101/gad.1986710
id	3947
pubmed_id	21123653

bacterial resistance :

Kanamycin

cloning :

backbone

pBIB-KAN-GW

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

used to create transgenic lines

growth notes :

and 5'-GATCCCTTGTGAATCCGAAGTAGTTGAGAGCAATGACAGTGCACTGTAGAAATCATGAGATTCATGG-3'). This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).
5'-CCATGAATCTCATGATTTCTACAGTGCACTGTCATT
GCTCTCAACTACTTCGGATTCACAAGGGATC-3'
Created by cloning 4.9-kb (including 1.8 kb upstream of CDS, CDS, and 1.6 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). The Quick- Change Lightning Site-Directed Mutagenesis kit (Stratagene) was used on the resulting plasmids to disrupt miR156-binding sites (primers

growth temp :

MACH-1 strain of E. coli (from Invitrogen)

origin :

pi :

alt_names

SPL10

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13 forward 20
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	F17L21.15, F17L21_15
gene	AT1G27370
id	839626

genbank_ids

mutation

miR156 target sites of SPL10 mutated

name

SPL10 mutant

shRNA_sequence

size

species

3702

Arabidopsis thaliana