This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSPL10p::SPL10-GFP
catalog :
27406
citations: 1
Reference
Nodine M, Bartel D. MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Genes Dev. 2010;24:2678-92 pubmed publisher
product information
Catalog Number :
27406
Product Name :
pSPL10p::SPL10-GFP
article :
doi10.1101/gad.1986710
id3947
pubmed_id21123653
bacterial resistance :
Kanamycin
cloning :
backboneKAN-GWR-GFP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
used to create transgenic lines
growth notes :
SPL10p::SPL10-GFP constructs were generated by cloning genomic fragments, including 1.7 kb upstream of and including the SPL10 coding sequence (CDS), into pENTR/D-TOPO (Invitrogen), and then recombining these plasmids with Gateway compatible pBIB-KAN-GWR-GFP plasmids using LR clonase (Invitrogen).
growth temp :
MACH-1 strain of E. coli (from Invitrogen)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3GFP Reverse
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesF17L21.15, F17L21_15
geneAT1G27370
id839626
genbank_ids
mutation
nameSPL10
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagGFP
resistance markers :
21
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA