This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBB541
catalog :
27394
citations: 3
Reference
Carabias A, Gómez Hernández M, de Cima S, Rodríguez Blázquez A, Morán Vaquero A, Gonzalez Saenz P, et al. Mechanisms of autoregulation of C3G, activator of the GTPase Rap1, and its catalytic deregulation in lymphomas. Sci Signal. 2020;13: pubmed publisher
Molugu S, Li J, Bernal R. Separation of E. coli chaperonin groEL from β-galactosidase without denaturation. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;1007:93-9 pubmed publisher
de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B. Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. BMC Biotechnol. 2007;7:32 pubmed
product information
Catalog Number :
27394
Product Name :
pBB541
article :
doi10.1186/1472-6750-7-32
id3949
pubmed_id17565681
bacterial resistance :
Spectinomycin
cloning :
backboneN/A
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb4143, ECK4137, groEL, mopA
genegroL
id948665
aliasesb4142, ECK4136, groES, mopB
genegroS
id948655
genbank_ids
mutation
namegroESL
shRNA_sequence
size
species
E. coli
tags
plasmid copy :
Dr. Bernd Bukau, ZMBH, University of Heidelberg
resistance markers :
879
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA