This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST/N1-hBIN1-GFP
catalog :
27305
citations: 3
Reference
Khurana H, Baratam K, Bhattacharyya S, Srivastava A, Pucadyil T. Mechanistic analysis of a novel membrane-interacting variable loop in the pleckstrin-homology domain critical for dynamin function. Proc Natl Acad Sci U S A. 2023;120:e2215250120 pubmed publisher
de la Mata A, Tajada S, O Dwyer S, Matsumoto C, Dixon R, Hariharan N, et al. BIN1 Induces the Formation of T-Tubules and Adult-Like Ca2+ Release Units in Developing Cardiomyocytes. Stem Cells. 2019;37:54-64 pubmed publisher
Hong T, Smyth J, Gao D, Chu K, Vogan J, Fong T, et al. BIN1 localizes the L-type calcium channel to cardiac T-tubules. PLoS Biol. 2010;8:e1000312 pubmed publisher
product information
Catalog Number :
27305
Product Name :
pDEST/N1-hBIN1-GFP
article :
doi10.1371/journal.pbio.1000312
id3924
pubmed_id20169111
bacterial resistance :
Kanamycin
cloning :
backbonepDEST/N1-GFP
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3attR2 gateway reaction site
cloning_site_5attR1 gateway reaction site
promoter
sequencing_primer_3
sequencing_primer_5sequence
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAMPH2, AMPHL, CNM2, SH3P9
geneBIN1
id274
genbank_ids
mutation
namebridging integrator 1 (BIN1), transcript variant 8
shRNA_sequence
size1362
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagGFP
plasmid copy :
We did not originally clone this gene. We ordered human BIN1 (transcript variance 8) from origene and cloned it into our gateway destination vector pDEST/N1-GFP. The N1-GFP vector is originally ordered from Clonetech and we converted it into a gateway destination vector.
resistance markers :
877
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA