This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Vinculin-venus
catalog :
27300
citations: 8
Reference
Chirasani V, Khan M, Malavade J, Dokholyan N, Hoffman B, Campbell S. Molecular basis and cellular functions of vinculin-actin directional catch bonding. Nat Commun. 2023;14:8300 pubmed publisher
Tao A, Lacroix A, Shoyer T, Venkatraman V, Xu K, Feiger B, et al. Identifying constitutive and context-specific molecular-tension-sensitive protein recruitment within focal adhesions. Dev Cell. 2023;58:522-534.e7 pubmed publisher
Campbell S, Chirasani V, Khan M, Malavade J, DOKHOLYAN N, Hoffman B. Elucidation of the Molecular Basis and Cellular Functions of Vinculin-Actin Directional Catch Bonding. Res Sq. 2023;: pubmed publisher
Marozas I, Anseth K, Cooper White J. Adaptable boronate ester hydrogels with tunable viscoelastic spectra to probe timescale dependent mechanotransduction. Biomaterials. 2019;223:119430 pubmed publisher
Ye A, Cane S, Maresca T. Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore. Nat Commun. 2016;7:13221 pubmed publisher
Vishavkarma R, Raghavan S, Kuyyamudi C, Majumder A, Dhawan J, Pullarkat P. Role of actin filaments in correlating nuclear shape and cell spreading. PLoS ONE. 2014;9:e107895 pubmed publisher
Burdyga A, Conant A, Haynes L, Zhang J, Jalink K, Sutton R, et al. cAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: effects of PKA and EPAC. Biochim Biophys Acta. 2013;1833:2664-2672 pubmed publisher
Grashoff C, Hoffman B, Brenner M, Zhou R, Parsons M, Yang M, et al. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature. 2010;466:263-6 pubmed publisher
product information
Catalog Number :
27300
Product Name :
Vinculin-venus
article :
doi10.1038/nature09198
id3586
pubmed_id20613844
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_origin
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVINC1
geneVCL
id396422
genbank_ids
NM_205441
mutation
nameVinculin
shRNA_sequence
size3927
species
9031
Gallus gallus
tags
locationC terminal on insert
tagVenus A206K
plasmid copy :
Chicken vinculin cDNA was originally isolated by Couto & Craig 1988 PNAS 85: 8535-8539
resistance markers :
451
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA