This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p3XFLAG-MKL1-~100
catalog :
27176
citations: 5
Reference
Le Dour C, Chatzifrangkeskou M, Macquart C, Magiera M, Peccate C, Jouve C, et al. Actin-microtubule cytoskeletal interplay mediated by MRTF-A/SRF signaling promotes dilated cardiomyopathy caused by LMNA mutations. Nat Commun. 2022;13:7886 pubmed publisher
Uetaki M, Onishi N, Oki Y, Shimizu T, Sugihara E, Sampetrean O, et al. Regulatory roles of fibronectin and integrin α5 in reorganization of the actin cytoskeleton and completion of adipogenesis. Mol Biol Cell. 2022;:mbcE21120609 pubmed publisher
Takahashi N, Nobusue H, Shimizu T, Sugihara E, Yamaguchi Iwai S, Onishi N, et al. ROCK Inhibition Induces Terminal Adipocyte Differentiation and Suppresses Tumorigenesis in Chemoresistant Osteosarcoma Cells. Cancer Res. 2019;79:3088-3099 pubmed publisher
Ronkina N, Lafera J, Kotlyarov A, Gaestel M. Stress-dependent phosphorylation of myocardin-related transcription factor A (MRTF-A) by the p38(MAPK)/MK2 axis. Sci Rep. 2016;6:31219 pubmed publisher
Cen B, Selvaraj A, Burgess R, Hitzler J, Ma Z, Morris S, et al. Megakaryoblastic leukemia 1, a potent transcriptional coactivator for serum response factor (SRF), is required for serum induction of SRF target genes. Mol Cell Biol. 2003;23:6597-608 pubmed
product information
Catalog Number :
27176
Product Name :
p3XFLAG-MKL1-~100
article :
doi10.1128/MCB.23.18.6597-6608.2003
id712
pubmed_id12944485
bacterial resistance :
Ampicillin
cloning :
backbonep3XFLAG-CMV7.1
backbone_mutation
backbone_originSigma
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Has no RPEL actin binding domain and is nuclear
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBSAC, MAL, MKL, MKL1, MRTF-A
geneMRTFA
id57591
genbank_ids
mutationDeletion of 100 aa from ATG
nameMKL1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag3XFLAG
resistance markers :
399
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA