pJM601-TRA-2::FLAG | Addgene 27129 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pJM601-TRA-2::FLAG

catalog :

27129

citations: 1

Reference
Mapes J, Chen J, Yu J, Xue D. Somatic sex determination in Caenorhabditis elegans is modulated by SUP-26 repression of tra-2 translation. Proc Natl Acad Sci U S A. 2010;107:18022-7 pubmed publisher

product information

Catalog Number :

27129

Product Name :

pJM601-TRA-2::FLAG

article :

doi	10.1073/pnas.1004513107
id	3874
pubmed_id	20921392

bacterial resistance :

Ampicillin

cloning :

backbone

pPD95.79

backbone_mutation

backbone_origin

backbone_size

2700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth notes :

Cloning of this plasmid was achieved in the following steps: (1)the sequence 3' (3'UTR) of the tags was added by (EcoRI/SpeI) (2) 1.2 kb XhoI/KpnI tra-2 pcr fragment into SalI/KpnI cut vector (destroying one SalI/XhoI site but adding another in the insert) immediately 5' of the tag (3) 7 kb HindIII/SalI fragment into same that partially replaced fragment added in step 2 containing most of the TRA-2 coding sequence (4) 5 kb Xho/AgeI that added the promoter and upstream gene in the operon as well as some Tra-2 coding sequence

origin :

pi :

alt_names

C15F1.3

cloning

clone_method	Restriction Enzyme
cloning_site_3	SpeI
cloning_site_5	XhoI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_C15F1.3
gene	tra-2
id	174137

genbank_ids

S42187

mutation

Replaced TRA-2 stop codon with KpnI and EcoRI and inserted 3 copies of the FLAG tag

name

TRA-2 FLAG

shRNA_sequence

size

13801

species

6239

Caenorhabditis elegans