eTC GFP -actin Zip | Addgene 27124 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

eTC GFP -actin Zip

catalog :

27124

citations: 4

Reference
Vedula P, Kurosaka S, MacTaggart B, Ni Q, Papoian G, Jiang Y, et al. Different translation dynamics of β- and γ-actin regulates cell migration. elife. 2021;10: pubmed publisher
Van Doorslaer K, Porter S, McKinney C, Stepp W, McBride A. Novel recombinant papillomavirus genomes expressing selectable genes. Sci Rep. 2016;6:37782 pubmed publisher
Akmatov M, Gatzemeier A, Schughart K, Pessler F. Equivalence of self- and staff-collected nasal swabs for the detection of viral respiratory pathogens. PLoS ONE. 2012;7:e48508 pubmed publisher
Rodriguez A, Shenoy S, Singer R, Condeelis J. Visualization of mRNA translation in living cells. J Cell Biol. 2006;175:67-76 pubmed

product information

Catalog Number :

27124

Product Name :

eTC GFP -actin Zip

article :

doi	10.1083/jcb.200512137
id	3870
pubmed_id	17030983

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Note: Addgene's sequencing results indicate the presence of several nucleotide inserts (starting at bp# 2459, 2501 and 2516) when compared to the sequence provided by the depositing laboratory. The depositing scientist confirmed that our sequencing result is correct and the inserts do not affect the function of the plasmid.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	HindIII
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
gene	ACTB
id	60

genbank_ids

mutation

This construct lacks -actin 3 UTR and therefore the zipcode sequence

name

beta-actin

shRNA_sequence

size

species

9606

Homo sapiens