eTC GFP beta-actin full length | Addgene 27123 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

eTC GFP beta-actin full length

catalog :

27123

citations: 7

Reference
Vedula P, Kurosaka S, MacTaggart B, Ni Q, Papoian G, Jiang Y, et al. Different translation dynamics of β- and γ-actin regulates cell migration. elife. 2021;10: pubmed publisher
Sharif T, Dai C, Martell E, Ghassemi Rad M, Hanes M, Murphy P, et al. TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem-Like Cells in a Redox-Sensitive Manner. Clin Cancer Res. 2019;25:2001-2017 pubmed publisher
Ramat A, Hannaford M, Januschke J. Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA. Curr Biol. 2017;27:2101-2111.e5 pubmed publisher
McKinney C, Kim M, Chen D, McBride A. Brd4 Activates Early Viral Transcription upon Human Papillomavirus 18 Infection of Primary Keratinocytes. MBio. 2016;7: pubmed publisher
Vedula P, Cruz L, Gutierrez N, Davis J, Ayee B, Abramczyk R, et al. Quantifying cadherin mechanotransduction machinery assembly/disassembly dynamics using fluorescence covariance analysis. Sci Rep. 2016;6:28822 pubmed publisher
Stepp W, Meyers J, McBride A. Sp100 provides intrinsic immunity against human papillomavirus infection. MBio. 2013;4:e00845-13 pubmed publisher
Rodriguez A, Shenoy S, Singer R, Condeelis J. Visualization of mRNA translation in living cells. J Cell Biol. 2006;175:67-76 pubmed

product information

Catalog Number :

27123

Product Name :

eTC GFP beta-actin full length

article :

doi	10.1083/jcb.200512137
id	3870
pubmed_id	17030983

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Note: Addgene's sequencing results indicate the presence of several nucleotide inserts (starting at bp# 2459, 2501 and 2516) and several gaps (at bp# 5352-5386) when compared to the sequence provided by the depositing laboratory. The depositing scientist confirmed that our sequencing result is correct and the differences do not affect the function of the plasmid. The plasmid map and sequence from the depositing laboratory lists EcoRI as the 3' cloning site; however, this site was destroyed during cloning. Please see the Addgene's sequencing results for cloning details. The beta-actin insert in the plasmids contains a E237A mutation when compared to GenBank reference sequences. This mutation was unintentional, but present in the initial clone. The plasmid functions as described in the associated publication.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	HindIII
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	T7
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
gene	ACTB
id	60

genbank_ids

mutation

E237A

name

beta-actin

shRNA_sequence

size

species

9606

Homo sapiens