This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCXLE-hMLN
catalog :
27079
citations: 9
Reference
Zywitza V, Rusha E, Shaposhnikov D, Ruiz Orera J, Telugu N, Rishko V, et al. Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction. Sci Rep. 2022;12:3100 pubmed publisher
Vermeer M, Bolling M, Bliley J, Arevalo Gomez K, Pavez Giani M, Kramer D, et al. Gain-of-function mutation in ubiquitin-ligase KLHL24 causes desmin degradation and dilatation in hiPSC-derived engineered heart tissues. J Clin Invest. 2021;: pubmed publisher
Bliley J, Vermeer M, Duffy R, Batalov I, Kramer D, Tashman J, et al. Dynamic loading of human engineered heart tissue enhances contractile function and drives a desmosome-linked disease phenotype. Sci Transl Med. 2021;13: pubmed publisher
Inzunza J, Arias Fuenzalida J, Segura Aguilar J, Nalvarte I, Varshney M. Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions. Stem Cell Res. 2021;51:102193 pubmed publisher
Wages P, Joshi P, Tallman K, Kim H, Bowman A, Porter N. Screening ToxCast™ for Chemicals That Affect Cholesterol Biosynthesis: Studies in Cell Culture and Human Induced Pluripotent Stem Cell-Derived Neuroprogenitors. Environ Health Perspect. 2020;128:17014 pubmed publisher
Simmons C, Thompson C, Cawthon B, Westlake G, Swoboda K, Kiskinis E, et al. Direct evidence of impaired neuronal Na/K-ATPase pump function in alternating hemiplegia of childhood. Neurobiol Dis. 2018;115:29-38 pubmed publisher
Kajiwara K, Tanemoto T, Wada S, Karibe J, Ihara N, Ikemoto Y, et al. Fetal Therapy Model of Myelomeningocele with Three-Dimensional Skin Using Amniotic Fluid Cell-Derived Induced Pluripotent Stem Cells. Stem Cell Reports. 2017;8:1701-1713 pubmed publisher
Tidball A, Neely M, Chamberlin R, Aboud A, Kumar K, Han B, et al. Genomic Instability Associated with p53 Knockdown in the Generation of Huntington's Disease Human Induced Pluripotent Stem Cells. PLoS ONE. 2016;11:e0150372 pubmed publisher
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011;8:409-12 pubmed publisher
product information
Catalog Number :
27079
Product Name :
pCXLE-hMLN
article :
doi10.1038/nmeth.1591
id4195
pubmed_id21460823
bacterial resistance :
Ampicillin
cloning :
backbonepCXLE
backbone_mutation
backbone_origin
backbone_size10180
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3WPRE-R
sequencing_primer_5pCAG-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutation
nameC-MYC, LIN28, NANOG
shRNA_sequence
size3104
species
9606
Homo sapiens
tags
plasmid copy :
pCEP4 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. Mutation(s) made by Provider lab: Three genes are connected with the foot-and-mousht desease virus 2A self-cleaving sequence.
resistance markers :
596
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA