This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pQCXIH-Flag Smad3 WT
catalog :
27025
citations: 3
Reference
Wolfson B, Zhang Y, Gernapudi R, Duru N, Yao Y, Lo P, et al. A High-Fat Diet Promotes Mammary Gland Myofibroblast Differentiation through MicroRNA 140 Downregulation. Mol Cell Biol. 2017;37: pubmed publisher
Nan L, Jacko A, Tan J, Wang D, Zhao J, KASS D, et al. Ubiquitin carboxyl-terminal hydrolase-L5 promotes TGFβ-1 signaling by de-ubiquitinating and stabilizing Smad2/Smad3 in pulmonary fibrosis. Sci Rep. 2016;6:33116 pubmed publisher
Gao S, Alarcon C, Sapkota G, Rahman S, Chen P, Goerner N, et al. Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Mol Cell. 2009;36:457-68 pubmed publisher
product information
Catalog Number :
27025
Product Name :
pQCXIH-Flag Smad3 WT
article :
doi10.1016/j.molcel.2009.09.043
id3866
pubmed_id19917253
bacterial resistance :
Ampicillin
cloning :
backbonepQCXIH
backbone_mutation
backbone_originClontech
backbone_size7800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
cDNA PCRed from pCS2-Flag Smad3 with 5' primer containing Flag tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3blunt end cloning
cloning_site_5blunt end cloning
promoter
sequencing_primer_3IRES-R
sequencing_primer_5LNCX
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
geneSMAD3
id4088
genbank_ids
mutation
nameSmad3
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
313
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA