This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI HA NEDD4
catalog :
27002
citations: 19
Reference
Xu L, Xiang W, Yang J, Gao J, Wang X, Meng L, et al. PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer. J Exp Clin Cancer Res. 2024;43:17 pubmed publisher
Marziali F, Song Y, Nguyen X, Belmudes L, Burlaud Gaillard J, Roingeard P, et al. A Proteomics-Based Approach Identifies the NEDD4 Adaptor NDFIP2 as an Important Regulator of Ifitm3 Levels. Viruses. 2023;15: pubmed publisher
Jiang X, Prabhakar A, van der Voorn S, Ghatpande P, Celona B, Venkataramanan S, et al. Control of ribosomal protein synthesis by the Microprocessor complex. Sci Signal. 2021;14: pubmed publisher
Hatstat A, Ahrendt H, Foster M, Mayne L, Moseley M, Englander S, et al. Characterization of Small-Molecule-Induced Changes in Parkinson's-Related Trafficking via the Nedd4 Ubiquitin Signaling Cascade. Cell Chem Biol. 2021;28:14-25.e9 pubmed publisher
Liu J, Yao Q, Xiao L, Ma W, Li F, Lai B, et al. PPARγ induces NEDD4 gene expression to promote autophagy and insulin action. FEBS J. 2020;287:529-545 pubmed publisher
Lee S, Park S, Lee H, Han S, Song J, Han D, et al. Nedd4 E3 ligase and beta-arrestins regulate ubiquitination, trafficking, and stability of the mGlu7 receptor. elife. 2019;8: pubmed publisher
Kim S, Ham S, Yang K, Kim K. Protein kinase CK2 activation is required for transforming growth factor β-induced epithelial-mesenchymal transition. Mol Oncol. 2018;12:1811-1826 pubmed publisher
Barber K, Muir P, Szeligowski R, Rogulina S, Gerstein M, Sampson J, et al. Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Nat Biotechnol. 2018;36:638-644 pubmed publisher
Tran M, Seo E, Min S, Nguyen Q, Choi J, Lee U, et al. NEDD4-induced degradative ubiquitination of phosphatidylinositol 4-phosphate 5-kinase ? and its implication in breast cancer cell proliferation. J Cell Mol Med. 2018;22:4117-4129 pubmed publisher
Pei G, Buijze H, Liu H, Moura Alves P, Goosmann C, Brinkmann V, et al. The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy. Autophagy. 2017;13:2041-2055 pubmed publisher
Suen K, Lin C, Seiler C, George R, Poncet Montange G, Biter A, et al. Phosphorylation of threonine residues on Shc promotes ligand binding and mediates crosstalk between MAPK and Akt pathways in breast cancer cells. Int J Biochem Cell Biol. 2018;94:89-97 pubmed publisher
Park H, Ryu D, Parmar M, Giasson B, McFarland N. The ER retention protein RER1 promotes alpha-synuclein degradation via the proteasome. PLoS ONE. 2017;12:e0184262 pubmed publisher
Totland M, Bergsland C, Fykerud T, Knudsen L, Rasmussen N, Eide P, et al. The E3 ubiquitin ligase NEDD4 induces endocytosis and lysosomal sorting of connexin 43 to promote loss of gap junctions. J Cell Sci. 2017;130:2867-2882 pubmed publisher
Liu J, Wan L, Liu J, Yuan Z, Zhang J, Guo J, et al. Cdh1 inhibits WWP2-mediated ubiquitination of PTEN to suppress tumorigenesis in an APC-independent manner. Cell Discov. 2016;2:15044 pubmed publisher
Noyes N, Hampton B, Migliorini M, Strickland D. Regulation of Itch and Nedd4 E3 Ligase Activity and Degradation by LRAD3. Biochemistry. 2016;55:1204-13 pubmed publisher
Chesarino N, McMichael T, Yount J. E3 Ubiquitin Ligase NEDD4 Promotes Influenza Virus Infection by Decreasing Levels of the Antiviral Protein IFITM3. PLoS Pathog. 2015;11:e1005095 pubmed publisher
Liu J, Wan L, Liu P, Inuzuka H, Liu J, Wang Z, et al. SCF(?-TRCP)-mediated degradation of NEDD4 inhibits tumorigenesis through modulating the PTEN/Akt signaling pathway. Oncotarget. 2014;5:1026-37 pubmed
Eide P, Cekaite L, Danielsen S, Eilertsen I, Kjenseth A, Fykerud T, et al. NEDD4 is overexpressed in colorectal cancer and promotes colonic cell growth independently of the PI3K/PTEN/AKT pathway. Cell Signal. 2013;25:12-8 pubmed publisher
Gao S, Alarcon C, Sapkota G, Rahman S, Chen P, Goerner N, et al. Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Mol Cell. 2009;36:457-68 pubmed publisher
product information
Catalog Number :
27002
Product Name :
pCI HA NEDD4
article :
doi10.1016/j.molcel.2009.09.043
id3866
pubmed_id19917253
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoter
sequencing_primer_3EBV-rev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEDD4-1, RPF1
geneNEDD4
id4734
genbank_ids
mutation
nameNEDD4
shRNA_sequence
size2700
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
313
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA