This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI HA NEDD4L
catalog :
27000
citations: 9
Reference
Wu T, Goriounova A, Worthington E, Wrennall J, Ghosh A, Ahmad S, et al. SPLUNC1 is a negative regulator of the Orai1 Ca2+ channel. Physiol Rep. 2022;10:e15306 pubmed publisher
Lavado García J, Díaz Maneh A, Canal Paulí N, Pérez Rubio P, Godia F, Cervera L. Metabolic engineering of HEK293 cells to improve transient transfection and cell budding of HIV-1 virus-like particles. Biotechnol Bioeng. 2021;: pubmed publisher
Morgenstern T, Park J, Fan Q, Colecraft H. A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits. elife. 2019;8: pubmed publisher
Guarnieri A, Towers C, Drasin D, Oliphant M, Andrysik Z, Hotz T, et al. The miR-106b-25 cluster mediates breast tumor initiation through activation of NOTCH1 via direct repression of NEDD4L. Oncogene. 2018;37:3879-3893 pubmed publisher
Kanner S, Morgenstern T, Colecraft H. Sculpting ion channel functional expression with engineered ubiquitin ligases. elife. 2017;6: pubmed publisher
Gwozdzinska P, Buchbinder B, Mayer K, Herold S, Morty R, Seeger W, et al. Hypercapnia Impairs ENaC Cell Surface Stability by Promoting Phosphorylation, Polyubiquitination and Endocytosis of ?-ENaC in a Human Alveolar Epithelial Cell Line. Front Immunol. 2017;8:591 pubmed publisher
Liu J, Wan L, Liu J, Yuan Z, Zhang J, Guo J, et al. Cdh1 inhibits WWP2-mediated ubiquitination of PTEN to suppress tumorigenesis in an APC-independent manner. Cell Discov. 2016;2:15044 pubmed publisher
Tanksley J, Chen X, Coffey R. NEDD4L is downregulated in colorectal cancer and inhibits canonical WNT signaling. PLoS ONE. 2013;8:e81514 pubmed publisher
Gao S, Alarcon C, Sapkota G, Rahman S, Chen P, Goerner N, et al. Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Mol Cell. 2009;36:457-68 pubmed publisher
product information
Catalog Number :
27000
Product Name :
pCI HA NEDD4L
article :
doi10.1016/j.molcel.2009.09.043
id3866
pubmed_id19917253
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoter
sequencing_primer_3EBV-rev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2
geneNEDD4L
id23327
genbank_ids
mutation
nameNEDD4L
shRNA_sequence
size3000
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
313
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA