This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCIBN(deltaNLS)-pmGFP
catalog :
26867
citations: 21
Reference
Pag xe8 s D, Dornier E, de Seze J, Gontran E, Maitra A, Maciejewski A, et al. Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments. Sci Adv. 2022;8:eabp8416 pubmed publisher
Wurz A, Bunner W, Szatmari E, Hughes R. CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures. J Biol Chem. 2022;298:102388 pubmed publisher
Qiu K, Zou W, Fang H, Hao M, Mehta K, Tian Z, et al. Light-activated mitochondrial fission through optogenetic control of mitochondria-lysosome contacts. Nat Commun. 2022;13:4303 pubmed publisher
Bérard M, Sheta R, Malvaut S, Rodriguez Aller R, Teixeira M, Idi W, et al. A light-inducible protein clustering system for in vivo analysis of α-synuclein aggregation in Parkinson disease. PLoS Biol. 2022;20:e3001578 pubmed publisher
Liao Z, Gingras A, Lagarrigue F, Ginsberg M, Shattil S. Optogenetics-based localization of talin to the plasma membrane promotes activation of β3 integrins. J Biol Chem. 2021;296:100675 pubmed publisher
Li T, Chen X, Qian Y, Shao J, Li X, Liu S, et al. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun. 2021;12:615 pubmed publisher
Yüz S, Rasoulinejad S, Mueller M, Wegner A, Wegner S. Blue Light Switchable Cell-Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom-Up Tissue Engineering. Adv Biosyst. 2019;3:e1800310 pubmed publisher
Perez Flores M, Lee J, Park S, Zhang X, Sihn C, Ledford H, et al. Cooperativity of Kv7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells. Sci Adv. 2020;6:eaba1104 pubmed publisher
Chen X, Zhang D, Su N, Bao B, Xie X, Zuo F, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37:1287-1293 pubmed publisher
O Banion C, Goswami A, Lawrence D. Design, construction, and validation of optogenetic proteins. Methods Enzymol. 2019;621:171-190 pubmed publisher
Liu Q, Sinnen B, Boxer E, Schneider M, Grybko M, Buchta W, et al. A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron. 2019;101:863-875.e6 pubmed publisher
Yüz S, Ricken J, Wegner S. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci (Weinh). 2018;5:1800446 pubmed publisher
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. Real-time observation of light-controlled transcription in living cells. J Cell Sci. 2017;130:4213-4224 pubmed publisher
O Banion C, Priestman M, Hughes R, Herring L, Capuzzi S, Lawrence D. Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. Cell Chem Biol. 2018;25:100-109.e8 pubmed publisher
Shiwarski D, Darr M, Telmer C, Bruchez M, Puthenveedu M. PI3K class II ? regulates ?-opioid receptor export from the trans-Golgi network. Mol Biol Cell. 2017;28:2202-2219 pubmed publisher
Lee D, Creed M, Jung K, Stefanelli T, Wendler D, Oh W, et al. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nat Methods. 2017;14:495-503 pubmed publisher
Guglielmi G, De Renzis S. Optogenetic inhibition of apical constriction during Drosophila embryonic development. Methods Cell Biol. 2017;139:167-186 pubmed publisher
Sinnen B, Bowen A, Forte J, Hiester B, Crosby K, Gibson E, et al. Optogenetic Control of Synaptic Composition and Function. Neuron. 2017;93:646-660.e5 pubmed publisher
Lainšček D, Lebar T, Jerala R. Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Biochem Biophys Res Commun. 2017;484:15-20 pubmed publisher
Xiong D, Xiao S, Guo S, Lin Q, Nakatsu F, Wu M. Frequency and amplitude control of cortical oscillations by phosphoinositide waves. Nat Chem Biol. 2016;12:159-66 pubmed publisher
Kennedy M, Hughes R, Peteya L, Schwartz J, Ehlers M, Tucker C. Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods. 2010;7:973-5 pubmed publisher
product information
Catalog Number :
26867
Product Name :
pCIBN(deltaNLS)-pmGFP
article :
doi10.1038/nmeth.1524
id3814
pubmed_id21037589
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane
origin :
37
pi :
alt_names
CIB1
cloning
clone_methodRestriction Enzyme
cloning_site_3Age I
cloning_site_5Nhe I
promoterCMV
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1
geneCIB1
id829604
genbank_ids
mutationNLS sequences mutated
nameCIBN(deltaNLS)-pmGFP
shRNA_sequence
size513
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagEGFP
resistance markers :
852
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA