This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCRY2PHR-mCherryN1
catalog :
26866
citations: 30
Reference
Yuan F, Gollapudi S, Day K, Ashby G, Sangani A, Malady B, et al. Ubiquitin-driven protein condensation promotes clathrin-mediated endocytosis. bioRxiv. 2023;: pubmed publisher
Pal D, Lin Y, Zhan H, Banerjee T, Kuhn J, Providence S, et al. Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movement. Front Cell Dev Biol. 2023;11:1195806 pubmed publisher
Boyd Shiwarski C, Shiwarski D, Griffiths S, Beacham R, Norrell L, Morrison D, et al. WNK kinases sense molecular crowding and rescue cell volume via phase separation. Cell. 2022;185:4488-4506.e20 pubmed publisher
Banerjee T, Biswas D, Pal D, Miao Y, Iglesias P, Devreotes P. Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration. Nat Cell Biol. 2022;24:1499-1515 pubmed publisher
Pag xe8 s D, Dornier E, de Seze J, Gontran E, Maitra A, Maciejewski A, et al. Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments. Sci Adv. 2022;8:eabp8416 pubmed publisher
Wurz A, Bunner W, Szatmari E, Hughes R. CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures. J Biol Chem. 2022;298:102388 pubmed publisher
Qiu K, Zou W, Fang H, Hao M, Mehta K, Tian Z, et al. Light-activated mitochondrial fission through optogenetic control of mitochondria-lysosome contacts. Nat Commun. 2022;13:4303 pubmed publisher
Kim G, Lee S, Jeong S, Lee J, Park D, Chang S. Multivalent electrostatic pi-cation interaction between synaptophysin and synapsin is responsible for the coacervation. Mol Brain. 2021;14:137 pubmed publisher
Herrera Perez R, Cupo C, Allan C, Lin A, Kasza K. Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extension. Biophys J. 2021;: pubmed publisher
Liao Z, Gingras A, Lagarrigue F, Ginsberg M, Shattil S. Optogenetics-based localization of talin to the plasma membrane promotes activation of β3 integrins. J Biol Chem. 2021;296:100675 pubmed publisher
Day K, Kago G, Wang L, Richter J, Hayden C, Lafer E, et al. Liquid-like protein interactions catalyse assembly of endocytic vesicles. Nat Cell Biol. 2021;23:366-376 pubmed publisher
Clarke J, Thibault P, Salapa H, Kim D, Hutchinson C, Levin M. Multiple Sclerosis-Associated hnRNPA1 Mutations Alter hnRNPA1 Dynamics and Influence Stress Granule Formation. Int J Mol Sci. 2021;22: pubmed publisher
Liu W, Irudayaraj J. Perfluorooctanoic acid (PFOA) exposure inhibits DNA methyltransferase activities and alters constitutive heterochromatin organization. Food Chem Toxicol. 2020;141:111358 pubmed publisher
Lamas I, Merlini L, Vjestica A, Vincenzetti V, Martin S. Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase. PLoS Biol. 2020;18:e3000600 pubmed publisher
Mann J, Gleixner A, Mauna J, Gomes E, DeChellis Marks M, Needham P, et al. RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43. Neuron. 2019;102:321-338.e8 pubmed publisher
McDaniel S, Gibson T, Schulz K, Fernandez Garcia M, Nevil M, Jain S, et al. Continued Activity of the Pioneer Factor Zelda Is Required to Drive Zygotic Genome Activation. Mol Cell. 2019;74:185-195.e4 pubmed publisher
Liu Q, Sinnen B, Boxer E, Schneider M, Grybko M, Buchta W, et al. A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron. 2019;101:863-875.e6 pubmed publisher
Putri R, Chen L. Spatiotemporal control of zebrafish (Danio rerio) gene expression using a light-activated CRISPR activation system. Gene. 2018;677:273-279 pubmed publisher
Kaur P, Saunders T, Tolwinski N. Coupling optogenetics and light-sheet microscopy, a method to study Wnt signaling during embryogenesis. Sci Rep. 2017;7:16636 pubmed publisher
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. Real-time observation of light-controlled transcription in living cells. J Cell Sci. 2017;130:4213-4224 pubmed publisher
O Banion C, Priestman M, Hughes R, Herring L, Capuzzi S, Lawrence D. Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. Cell Chem Biol. 2018;25:100-109.e8 pubmed publisher
Huang A, Amourda C, Zhang S, Tolwinski N, Saunders T. Decoding temporal interpretation of the morphogen Bicoid in the early Drosophila embryo. elife. 2017;6: pubmed publisher
Lee D, Creed M, Jung K, Stefanelli T, Wendler D, Oh W, et al. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nat Methods. 2017;14:495-503 pubmed publisher
Guglielmi G, De Renzis S. Optogenetic inhibition of apical constriction during Drosophila embryonic development. Methods Cell Biol. 2017;139:167-186 pubmed publisher
Sinnen B, Bowen A, Forte J, Hiester B, Crosby K, Gibson E, et al. Optogenetic Control of Synaptic Composition and Function. Neuron. 2017;93:646-660.e5 pubmed publisher
Lainšček D, Lebar T, Jerala R. Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Biochem Biophys Res Commun. 2017;484:15-20 pubmed publisher
Gao Y, Xiong X, Wong S, Charles E, Lim W, Qi L. Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016;13:1043-1049 pubmed publisher
Choudhury S, Cui Y, Narayanan A, Gilley D, Huda N, Lo C, et al. Optogenetic regulation of site-specific subtelomeric DNA methylation. Oncotarget. 2016;7:50380-50391 pubmed publisher
O Neill P, Gautam N. Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell. 2014;25:2305-14 pubmed publisher
Kennedy M, Hughes R, Peteya L, Schwartz J, Ehlers M, Tucker C. Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods. 2010;7:973-5 pubmed publisher
product information
Catalog Number :
26866
Product Name :
pCRY2PHR-mCherryN1
article :
doi10.1038/nmeth.1524
id3814
pubmed_id21037589
bacterial resistance :
Kanamycin
cloning :
backbonepmCherryN1
backbone_mutation
backbone_originClontech
backbone_size4722
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules
origin :
37
pi :
alt_names
cryptochrome 2
cloning
clone_methodRestriction Enzyme
cloning_site_3Xma I
cloning_site_5Xho I
promoterCMV
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
geneCRY2
id839529
genbank_ids
mutation
nameCRY2PHR
shRNA_sequence
size1507
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagmCherry
resistance markers :
852
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA