ninaE-dsocs | Addgene 26857 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

ninaE-dsocs

catalog :

26857

citations: 1

Reference
Wang T, Wang X, Xie Q, Montell C. The SOCS box protein STOPS is required for phototransduction through its effects on phospholipase C. Neuron. 2008;57:56-68 pubmed publisher

product information

Catalog Number :

26857

Product Name :

ninaE-dsocs

article :

doi	10.1016/j.neuron.2007.11.020
id	3792
pubmed_id	18184564

bacterial resistance :

Ampicillin

cloning :

backbone

pCNX

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

To express the stops cDNA under the control of the ninaE promoter, the cDNA (EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites of pCNX. Myc-tagged STOPS lacking the SOCS box was subcloned between the NotI and XbaI sites of pCNX. The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector

origin :

pi :

alt_names

STOPS

CG31006

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	NotI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CG15557, CG15558, CG15559, CG31006, Dmel CG31006, su(rdgB)69
gene	stops
id	43683

genbank_ids

CG31006

mutation

This STOPS construct contains a mutated/truncated SOCS box (suppressor of cytokine signaling). Amino acids 296 and 297 have been mutated from SL to EF and amino acids 298-330 have been deleted

name

Slow Termination of Phototransduction

shRNA_sequence

size

species

7227

Drosophila melanogaster