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product summary
company name :
Addgene
product type :
cDNA
product name :
ninaE-stops m3-myc
catalog :
26855
citations: 1
product information
Catalog Number :
26855
Product Name :
ninaE-stops m3-myc
article :
| doi | 10.1016/j.neuron.2007.11.020 |
| id | 3792 |
| pubmed_id | 18184564 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pCNX | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
To express the stops cDNA under the control of the ninaE promoter, the cDNA (EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end of the wild-type stops cDNA. The wild-type stops cDNAs was subsequently subcloned between the NotI and XbaI sites of pCNX. The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
origin :
37
pi :
|
resistance markers :
| 346 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments