pcDNA3.3_c-MYC | Addgene 26818 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.3_c-MYC

catalog :

26818

citations: 9

Reference
Sun Q, Shi L, Li S, Li J, Zhang R, Huang X, et al. PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis. Free Radic Biol Med. 2023;205:13-24 pubmed publisher
Shan L, Liu W, Zhan Y. Long Non-coding RNA CCAT1 Acts as an Oncogene and Promotes Sunitinib Resistance in Renal Cell Carcinoma. Front Oncol. 2020;10:516552 pubmed publisher
Dong H, Hu J, Wang L, Qi M, Lu N, Tan X, et al. SOX4 is activated by C-MYC in prostate cancer. Med Oncol. 2019;36:92 pubmed publisher
McGrath P, Diette N, Kogut I, Bilousova G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J Vis Exp. 2018;: pubmed publisher
Soleymani Abyaneh H, Gupta N, Alshareef A, Gopal K, Lavasanifar A, Lai R. Hypoxia Induces the Acquisition of Cancer Stem-like Phenotype Via Upregulation and Activation of Signal Transducer and Activator of Transcription-3 (STAT3) in MDA-MB-231, a Triple Negative Breast Cancer Cell Line. Cancer Microenviron. 2018;11:141-152 pubmed publisher
Kogut I, McCarthy S, Pavlova M, Astling D, Chen X, Jakimenko A, et al. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018;9:745 pubmed publisher
Gupta N, Jung K, Wu C, Alshareef A, Alqahtani H, Damaraju S, et al. High Myc expression and transcription activity underlies intra-tumoral heterogeneity in triple-negative breast cancer. Oncotarget. 2017;8:28101-28115 pubmed publisher
Wang G, McCain M, Yang L, He A, Pasqualini F, Agarwal A, et al. Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies. Nat Med. 2014;20:616-23 pubmed publisher
Warren L, Manos P, Ahfeldt T, Loh Y, Li H, Lau F, et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 2010;7:618-30 pubmed publisher

product information

Catalog Number :

26818

Product Name :

pcDNA3.3_c-MYC

article :

doi	10.1016/j.stem.2010.08.012
id	3789
pubmed_id	20888316

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.3

backbone_mutation

backbone_origin

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

AGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATG The 3' UTR sequence is: GCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCT CCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAA GTGAGGGTCTAGAACTAGTGTCGACGC
TGGACCCTCGTACAGAAGCTAATACGACTCACTATAGGG
AAATAAGAG
Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine. Comment from lab: with reference to NM_002467.4 sequence we have two mutations at following positions: 172 (A to G) and 174 (C to G) there is some confusion as to what the ref seq is exactly but these are the constructs that we've been using successfully. Please note that the 5'UTR and 3'UTR sequences are synthetic and were added to the c-MYC ORF as described in Figure S1 and Table S2 of Warren et al., Cell Stem Cell. 2010. These UTR sequences are not endogenous c-MYC UTR sequences. The 5' UTR sequence (including T7 promoter) is:

origin :

pi :

alt_names

c-myc

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	TK polyA reverse
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

Sequence begins from second ATG; Synthetic UTR sequences added to c-MYC ORF

name

5'UTR-c-MYC-3'UTR

shRNA_sequence

size

1504

species

9606

Homo sapiens