pcDNA3.3_OCT4 | Addgene 26816 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.3_OCT4

catalog :

26816

citations: 3

Reference
Kogut I, McCarthy S, Pavlova M, Astling D, Chen X, Jakimenko A, et al. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018;9:745 pubmed publisher
Hattermann K, Flüh C, Engel D, Mehdorn H, Synowitz M, Mentlein R, et al. Stem cell markers in glioma progression and recurrence. Int J Oncol. 2016;49:1899-1910 pubmed publisher
Warren L, Manos P, Ahfeldt T, Loh Y, Li H, Lau F, et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 2010;7:618-30 pubmed publisher

product information

Catalog Number :

26816

Product Name :

pcDNA3.3_OCT4

article :

doi	10.1016/j.stem.2010.08.012
id	3789
pubmed_id	20888316

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.3

backbone_mutation

backbone_origin

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Note that OCT4 has some silent mutations. Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.

origin :

pi :

alt_names

OCT4

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	TK polyA reverse
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

mutation

name

5'UTR-OCT4-3'UTR

shRNA_sequence

size

1266

species

9606

Homo sapiens