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product summary
company name :
Addgene
product type :
cDNA
product name :
pEGIP
catalog :
26777
citations: 32
Reference
Lasota S, Zimoląg E, Bobis Wozowicz S, Pilipiuk J, Madeja Z. The dynamics of the electrotactic reaction of mouse 3T3 fibroblasts. Biochim Biophys Acta Mol Cell Res. 2024;1871:119647 pubmed publisher
Schoen A, H xf6 lzer M, M xfc ller M, Wallerang K, Drosten C, Marz M, et al. Functional comparisons of the virus sensor RIG-I from humans, the microbat Myotis daubentonii, and the megabat Rousettus aegyptiacus, and their response to SARS-CoV-2 infection. J Virol. 2023;97:e0020523 pubmed publisher
de Ara xfa jo Rolo C, Machado B, Dos Santos M, Dos Santos R, Fonseca M, Hodel K, et al. Long-term monitoring of COVID-19 prevalence in raw and treated wastewater in Salvador, the largest capital of the Brazilian Northeast. Sci Rep. 2023;13:15238 pubmed publisher
Halawani D, Wang Y, Ramakrishnan A, Estill M, He X, Shen L, et al. Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons. Nat Commun. 2023;14:5165 pubmed publisher
Kumar A, He S, Mali P. Systematic discovery of transcription factors that improve hPSC-derived cardiomyocyte maturation via temporal analysis of bioengineered cardiac tissues. APL Bioeng. 2023;7:026109 pubmed publisher
Duman C, Di Marco B, Nevedomskaya E, Ulug B, Lesche R, Christian S, et al. Targeting fatty acid oxidation via Acyl-CoA binding protein hinders glioblastoma invasion. Cell Death Dis. 2023;14:296 pubmed publisher
Sim J, Shin D, Park P, Park S, Bae J, Lee Y, et al. Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition. Cell Rep. 2022;40:111117 pubmed publisher
Ageely E, Chilamkurthy R, Jana S, Abdullahu L, O Reilly D, Jensik P, et al. Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles. Nat Commun. 2021;12:6591 pubmed publisher
Generali M, Satheesha S, Bode P, Wanner D, Schafer B, Casanova E. High Frequency of Tumor Propagating Cells in Fusion-Positive Rhabdomyosarcoma. Genes (Basel). 2021;12: pubmed publisher
Ford K, Panwala R, Chen D, Portell A, Palmer N, Mali P. Peptide-tiling screens of cancer drivers reveal oncogenic protein domains and associated peptide inhibitors. Cell Syst. 2021;12:716-732.e7 pubmed publisher
Jiang M, Yu S, Wei Z, Zhong W, Cao W, Gu X, et al. Conversion of Reactive Astrocytes to Induced Neurons Enhances Neuronal Repair and Functional Recovery After Ischemic Stroke. Front Aging Neurosci. 2021;13:612856 pubmed publisher
Huang S, Bruce T, Ding H, Wei Y, Marcus R. Rapid isolation of lentivirus particles from cell culture media via a hydrophobic interaction chromatography method on a polyester, capillary-channeled polymer fiber stationary phase. Anal Bioanal Chem. 2021;413:2985-2994 pubmed publisher
Lukonin I, Serra D, Challet Meylan L, Volkmann K, Baaten J, Zhao R, et al. Phenotypic landscape of intestinal organoid regeneration. Nature. 2020;586:275-280 pubmed publisher
Santos G, Silva D, Fortuna V, Silveira B, Orge I, de Santana T, et al. Leukemia Inhibitory Factor (LIF) Overexpression Increases the Angiogenic Potential of Bone Marrow Mesenchymal Stem/Stromal Cells. Front Cell Dev Biol. 2020;8:778 pubmed publisher
Ferreira Mendes J, de Faro Valverde L, Torres Andion Vidal M, Paredes B, Coelho P, Allahdadi K, et al. Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma. Int J Mol Sci. 2020;21: pubmed publisher
Orge I, Gadd V, Barouh J, Rossi E, Carvalho R, Smith I, et al. Phenotype instability of hepatocyte-like cells produced by direct reprogramming of mesenchymal stromal cells. Stem Cell Res Ther. 2020;11:154 pubmed publisher
Hart P, Kenny H, Grassl N, Watters K, Litchfield L, Coscia F, et al. Mesothelial Cell HIF1α Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk. Cell Rep. 2019;29:4086-4098.e6 pubmed publisher
Nakanishi M, Mitchell R, Benoit Y, Orlando L, Reid J, Shimada K, et al. Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell. 2019;177:910-924.e22 pubmed publisher
Daviaud N, Friedel R, Zou H. Vascularization and Engraftment of Transplanted Human Cerebral Organoids in Mouse Cortex. Eneuro. 2018;5: pubmed publisher
Savic N, Ringnalda F, Lindsay H, Berk C, Bargsten K, Li Y, et al. Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair. elife. 2018;7: pubmed publisher
Yin H, Song C, Suresh S, Kwan S, Wu Q, Walsh S, et al. Partial DNA-guided Cas9 enables genome editing with reduced off-target activity. Nat Chem Biol. 2018;14:311-316 pubmed publisher
Chau M, Deveau T, Song M, Wei Z, Gu X, Yu S, et al. Transplantation of iPS cell-derived neural progenitors overexpressing SDF-1? increases regeneration and functional recovery after ischemic stroke. Oncotarget. 2017;8:97537-97553 pubmed publisher
Gonçalves G, Silva D, Carvalho R, Souza B, da Silva K, Vasconcelos J, et al. Generation and characterization of transgenic mouse mesenchymal stem cell lines expressing hIGF-1 or hG-CSF. Cytotechnology. 2018;70:577-591 pubmed publisher
Wei Z, Zhang J, Taylor T, Gu X, Zhao Y, Wei L. Neuroprotective and regenerative roles of intranasal Wnt-3a administration after focal ischemic stroke in mice. J Cereb Blood Flow Metab. 2018;38:404-421 pubmed publisher
Wu J, Platero Luengo A, Sakurai M, Sugawara A, Gil M, Yamauchi T, et al. Interspecies Chimerism with Mammalian Pluripotent Stem Cells. Cell. 2017;168:473-486.e15 pubmed publisher
Xue Z, Hennelly S, Doyle B, Gulati A, Novikova I, Sanbonmatsu K, et al. A G-Rich Motif in the lncRNA Braveheart Interacts with a Zinc-Finger Transcription Factor to Specify the Cardiovascular Lineage. Mol Cell. 2016;64:37-50 pubmed publisher
Tapia O, Gerace L. Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation. Methods Mol Biol. 2016;1411:177-94 pubmed publisher
Yin H, Song C, Dorkin J, Zhu L, Li Y, Wu Q, et al. Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo. Nat Biotechnol. 2016;34:328-33 pubmed publisher
Alves C, Dariolli R, Jorge F, Monteiro M, Maximino J, Martins R, et al. Gene expression profiling for human iPS-derived motor neurons from sporadic ALS patients reveals a strong association between mitochondrial functions and neurodegeneration. Front Cell Neurosci. 2015;9:289 pubmed publisher
Byrne S, Ortiz L, Mali P, Aach J, Church G. Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells. Nucleic Acids Res. 2015;43:e21 pubmed publisher
Mali P, Aach J, Stranges P, Esvelt K, Moosburner M, Kosuri S, et al. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013;31:833-8 pubmed publisher
Zou J, Maeder M, Mali P, Pruett Miller S, Thibodeau Beganny S, Chou B, et al. Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Cell Stem Cell. 2009;5:97-110 pubmed publisher
product information
Catalog Number :
26777
Product Name :
pEGIP
article :
doi10.1016/j.stem.2009.05.023
id3774
pubmed_id19540188
bacterial resistance :
Ampicillin
cloning :
backboneDuet011
backbone_mutation
backbone_origin
backbone_size7063
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Allows for puromycin selection of stable integration of GFP expression cassette. With modification, this vector enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting.
growth strain :
Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.
origin :
37
pi :
alt_names
GFP
Puromycin resistant gene
EF1alpha promoter
cloning
clone_methodRestriction Enzyme
cloning_site_3HpaI
cloning_site_5PacI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasespCmGFP_001
genegfp
id7011691
genbank_ids
mutation
nameEF1a-GFP-IRES-Puro
shRNA_sequence
size3229
species
tags
resistance markers :
76
tags :
High Copy
terms :
Puromycin
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA