This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDREV-2
catalog :
26752
citations: 1
Reference
Osterwalder M, Galli A, Rosen B, Skarnes W, Zeller R, Lopez Rios J. Dual RMCE for efficient re-engineering of mouse mutant alleles. Nat Methods. 2010;7:893-5 pubmed publisher
product information
Catalog Number :
26752
Product Name :
pDREV-2
article :
doi10.1038/nmeth.1521
id3759
pubmed_id20953177
bacterial resistance :
Nourseothricin (clonNat)
cloning :
backbonepL1L2_gt2
backbone_mutation
backbone_originB. Rosen and W.C.Skarnes, unpublished
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
Other
FLP/FRT; DRE/Rox
origin :
37
pi :
alt_names
HIST1H2BB, H2B.1, H2B/f, H2BFF, MGC119804
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BlgII
promoter
sequencing_primer_3
sequencing_primer_5FCHK2 sequence: GTATCTGCAACCTCAAGCTAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B.1, H2B/f, H2BFF, HIST1H2BB
geneH2BC3
id3018
genbank_ids
NM_021058
mutation
nameH2B-Venus CDS
shRNA_sequence
size1101
species
9606
Homo sapiens
tags
locationC terminal on insert
tagVenus
plasmid copy :
PGK-Puro cassette from Addgene plasmid 11349
resistance markers :
836
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA