This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-rGBD
catalog :
26732
citations: 12
Reference
Michaud A, Leda M, Swider Z, Kim S, He J, Landino J, et al. A versatile cortical pattern-forming circuit based on Rho, F-actin, Ect2, and RGA-3/4. J Cell Biol. 2022;221: pubmed publisher
Mahlandt E, Arts J, van der Meer W, van der Linden F, Tol S, van Buul J, et al. Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor. J Cell Sci. 2021;: pubmed publisher
Pal D, Ellis A, Sepúlveda Ramírez S, Salgado T, Terrazas I, Reyes G, et al. Rac and Arp2/3-Nucleated Actin Networks Antagonize Rho During Mitotic and Meiotic Cleavages. Front Cell Dev Biol. 2020;8:591141 pubmed publisher
Streets A, Prosseda P, Ong A. Polycystin-1 regulates ARHGAP35-dependent centrosomal RhoA activation and ROCK signalling. JCI Insight. 2020;: pubmed publisher
Poirier M, Fiorino C, Rajasekar T, Harrison R. F-actin flashes on phagosomes mechanically deform contents for efficient digestion in macrophages. J Cell Sci. 2020;133: pubmed publisher
Hori A, Nishide K, Yasukuni Y, Haga K, Kakuta W, Ishikawa Y, et al. Prominin-1 Modulates Rho/ROCK-Mediated Membrane Morphology and Calcium-Dependent Intracellular Chloride Flux. Sci Rep. 2019;9:15911 pubmed publisher
DeKraker C, Goldin Blais L, Boucher E, Mandato C. Dynamics of actin polymerisation during the mammalian single-cell wound healing response. BMC Res Notes. 2019;12:420 pubmed publisher
O Neill P, Castillo Badillo J, Meshik X, Kalyanaraman V, Melgarejo K, Gautam N. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell. 2018;46:9-22.e4 pubmed publisher
Li C, MacDonald J, Talebian A, Leuenberger J, Seah C, Pasternak S, et al. Unravelling the Mechanism of TrkA-Induced Cell Death by Macropinocytosis in Medulloblastoma Daoy Cells. Mol Cell Biol. 2016;36:2596-611 pubmed publisher
Kourtidis A, Ngok S, Pulimeno P, Feathers R, Carpio L, Baker T, et al. Distinct E-cadherin-based complexes regulate cell behaviour through miRNA processing or Src and p120 catenin activity. Nat Cell Biol. 2015;17:1145-57 pubmed publisher
Miklavc P, Ehinger K, Sultan A, Felder T, PAUL P, Gottschalk K, et al. Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles. J Cell Sci. 2015;128:1193-203 pubmed publisher
Benink H, Bement W. Concentric zones of active RhoA and Cdc42 around single cell wounds. J Cell Biol. 2005;168:429-39 pubmed
product information
Catalog Number :
26732
Product Name :
GFP-rGBD
article :
doi10.1083/jcb.200411109
id3753
pubmed_id15684032
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size4097
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
reporter for Rho
growth notes :
The RhoA-binding domain of rhotekin (rGBD) was fused to eGFP in order to visualize active RhoA
origin :
37
pi :
alt_names
rGBD
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5XhoI
promoter
sequencing_primer_3T7
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesplekhk1
genertkn2
id495119
genbank_ids
mutationRhoA binding domain of rhotekin
namerhotekin
shRNA_sequence
size249
species
10090
Mus musculus
tags
locationN terminal on insert
tagGFP
plasmid copy :
GST-rGBD construc was provided by K. Burridge (University of North Carolina at Chapel Hill)
resistance markers :
840
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA