This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO.1-puro-cfSec15A_5
catalog :
26716
citations: 1
Reference
Bryant D, Datta A, Rodriguez Fraticelli A, Peranen J, Martin Belmonte F, Mostov K. A molecular network for de novo generation of the apical surface and lumen. Nat Cell Biol. 2010;12:1035-45 pubmed publisher
product information
Catalog Number :
26716
Product Name :
pLKO.1-puro-cfSec15A_5
article :
doi10.1038/ncb2106
id3732
pubmed_id20890297
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1-puro
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
RNAi only matches dog Sec15A, does not match Sec15B. Does not match other species' Sec15A. RNAi target sequence is: 5'-GCACGGGTCATGATAGTTT-3'.
origin :
37
pi :
alt_names
EXOC6
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesSEC15A, SEC15L, SEC15L1
geneEXOC6
id477771
genbank_ids
mutationDog Sec15A-specific RNAi.
nameDog Sec15A RNAi
shRNA_sequenceGCACGGGTCATGATAGTTT
size
species
32644
Other
C. familiaris (dog RNAi)
tags
resistance markers :
830
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA