p1242 3x-KB-L | Addgene 26699 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p1242 3x-KB-L

catalog :

26699

citations: 15

Reference
Koeberle S, Gollowitzer A, Laoukili J, Kranenburg O, Werz O, Koeberle A, et al. Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-κB-driven inflammation through redox-active mechanisms. Redox Biol. 2020;28:101388 pubmed publisher
Francisco J, Byun J, Zhang Y, Kalloo O, Mizushima W, Oka S, et al. The tumor suppressor RASSF1A modulates inflammation and injury in the reperfused murine myocardium. J Biol Chem. 2019;294:13131-13144 pubmed publisher
Keklikoglou I, Cianciaruso C, Güç E, Squadrito M, Spring L, Tazzyman S, et al. Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. Nat Cell Biol. 2019;21:190-202 pubmed publisher
Hsu R, Zhong C, Wang C, Liao W, Yang C, Lin S, et al. Golgi tethering factor golgin-97 suppresses breast cancer cell invasiveness by modulating NF-κB activity. Cell Commun Signal. 2018;16:19 pubmed publisher
Fusella F, Seclì L, Busso E, Krepelova A, Moiso E, Rocca S, et al. The IKK/NF-κB signaling pathway requires Morgana to drive breast cancer metastasis. Nat Commun. 2017;8:1636 pubmed publisher
Willson J, Muir C, Evered C, Cepeda M, Damjanovski S. Stable expression of ?1-antitrypsin Portland in MDA-MB-231 cells increased MT1-MMP and MMP-9 levels, but reduced tumour progression. J Cell Commun Signal. 2018;12:479-488 pubmed publisher
Liliom H, Tárnok K, Ábrahám Z, Racz B, Hausser A, Schlett K. Protein kinase D exerts neuroprotective functions during oxidative stress via nuclear factor kappa B-independent signaling pathways. J Neurochem. 2017;142:948-961 pubmed publisher
Cepeda M, Evered C, Pelling J, Damjanovski S. Inhibition of MT1-MMP proteolytic function and ERK1/2 signalling influences cell migration and invasion through changes in MMP-2 and MMP-9 levels. J Cell Commun Signal. 2017;11:167-179 pubmed publisher
Cepeda M, Pelling J, Evered C, Leong H, Damjanovski S. The cytoplasmic domain of MT1-MMP is dispensable for migration augmentation but necessary to mediate viability of MCF-7 breast cancer cells. Exp Cell Res. 2017;350:169-183 pubmed publisher
Li L, Chen J, Xiong G, St Clair D, Xu W, Xu R. Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture. J Cell Sci. 2017;130:190-202 pubmed publisher
Yamaguchi K, Takanashi T, Nasu K, Tamai K, Mochizuki M, Satoh I, et al. Xenotransplantation elicits salient tumorigenicity of adult T-cell leukemia-derived cells via aberrant AKT activation. Cancer Sci. 2016;107:638-43 pubmed publisher
Cott C, Thuenauer R, Landi A, Kühn K, Juillot S, Imberty A, et al. Pseudomonas aeruginosa lectin LecB inhibits tissue repair processes by triggering β-catenin degradation. Biochim Biophys Acta. 2016;1863:1106-18 pubmed publisher
Lambert A, Wong C, Ozturk S, Papageorgis P, Raghunathan R, Alekseyev Y, et al. Tumor Cell-Derived Periostin Regulates Cytokines That Maintain Breast Cancer Stem Cells. Mol Cancer Res. 2016;14:103-13 pubmed publisher
Park E, Min K, Choi K, Kwon T. Dicoumarol sensitizes renal cell carcinoma Caki cells to TRAIL-induced apoptosis through down-regulation of Bcl-2, Mcl-1 and c-FLIP in a NQO1-independent manner. Exp Cell Res. 2014;323:144-54 pubmed publisher
Mitchell T, Sugden B. Stimulation of NF-kappa B-mediated transcription by mutant derivatives of the latent membrane protein of Epstein-Barr virus. J Virol. 1995;69:2968-76 pubmed

product information

Catalog Number :

26699

Product Name :

p1242 3x-KB-L

article :

doi
id	3744
pubmed_id	7707523

bacterial resistance :

Ampicillin

cloning :

backbone

pGL2-Basic

backbone_mutation

backbone_origin

Promega

backbone_size

5600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII).

origin :

pi :

alt_names

MHC I NFKB element

luciferase

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	SmaI
promoter
sequencing_primer_3
sequencing_primer_5	EBV Reverse
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB
gene	NFKB1
id	4790

genbank_ids

mutation

name

3x MHC I KB element - luciferase

shRNA_sequence

size

200

species

9606

Homo sapiens

Photinus pyralis