This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRL-TK 3'FBXW7 UTR
catalog :
26649
citations: 2
Reference
Fu Q, Lu Z, Fu X, Ma S, Lu X. MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway. Aging (Albany NY). 2019;11:11865-11879 pubmed publisher
Xu Y, Sengupta T, Kukreja L, Minella A. MicroRNA-223 regulates cyclin E activity by modulating expression of F-box and WD-40 domain protein 7. J Biol Chem. 2010;285:34439-46 pubmed publisher
product information
Catalog Number :
26649
Product Name :
pRL-TK 3'FBXW7 UTR
article :
doi10.1074/jbc.M110.152306
id3729
pubmed_id20826802
bacterial resistance :
Ampicillin
cloning :
backbonepRL-TK
backbone_mutation
backbone_originPromega
backbone_size4045
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
Please note that Addgene's sequencing results identified nucleotide mismatches when compared to GenBank reference sequence NM_018315.4 at basepair #'s 2389, 2663, 2666, 2829 and 3526.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5XbaI
promoter
sequencing_primer_3EBV-Rev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
geneFBXW7
id55294
genbank_ids
mutationContains only the 3'UTR of FBXW7, nucleotides 1-1571 following stop codon.
nameFBXW7
shRNA_sequence
size1571
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagLuciferase
resistance markers :
821
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA