pLJM1 p18 mito | Addgene 26638 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLJM1 p18 mito

catalog :

26638

citations: 1

Reference
Sancak Y, Bar Peled L, Zoncu R, Markhard A, Nada S, Sabatini D. Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Cell. 2010;141:290-303 pubmed publisher

product information

Catalog Number :

26638

Product Name :

pLJM1 p18 mito

article :

doi	10.1016/j.cell.2010.02.024
id	3721
pubmed_id	20381137

bacterial resistance :

Ampicillin

cloning :

backbone

pLJM1

backbone_mutation

backbone_origin

Available at Addgene (#19319)

backbone_size

7300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The p18 mito expression plasmid was generated by cloning of a mutant p18 with amino acids 2 4 changed to alanines into a modi ed version of the pLKO.1 vector that added, to the C terminus of p18, the mitochondrial localization signal of OMP25 protein. The order of the construct is the following: Age1-flag-sal-p18(residues 2-4 mutated to alanine, stop condon removed)-GGGGS (2X polylinker)-omp25 seq-stop codon-ecor1

growth temp :

Grow in XL10Gold or Stbl3 cells to decrease recombination. Growing in SOC also helps.

origin :

pi :

alt_names

p18

c11orf59

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C11orf59, PDRO, Ragulator1, p18, p27RF-Rho
gene	LAMTOR1
id	55004

genbank_ids

mutation

name

p18 mito

shRNA_sequence

size

species

9606

Homo sapiens