pLJM1 Flag Raptor HRas25 | Addgene 26637 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLJM1 Flag Raptor HRas25

catalog :

26637

citations: 3

Reference
Norambuena A, Wallrabe H, Cao R, Wang D, Silva A, Švindrych Z, et al. A novel lysosome-to-mitochondria signaling pathway disrupted by amyloid-β oligomers. EMBO J. 2018;37: pubmed publisher
Norambuena A, Wallrabe H, McMahon L, Silva A, Swanson E, Khan S, et al. mTOR and neuronal cell cycle reentry: How impaired brain insulin signaling promotes Alzheimer's disease. Alzheimers Dement. 2017;13:152-167 pubmed publisher
Sancak Y, Bar Peled L, Zoncu R, Markhard A, Nada S, Sabatini D. Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Cell. 2010;141:290-303 pubmed publisher

product information

Catalog Number :

26637

Product Name :

pLJM1 Flag Raptor HRas25

article :

doi	10.1016/j.cell.2010.02.024
id	3721
pubmed_id	20381137

bacterial resistance :

Ampicillin

cloning :

backbone

pLJM1

backbone_mutation

backbone_origin

Available at Addgene (#19319)

backbone_size

7400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The insert is structured: AgeI-FlagTag-SalI-Raptor without stop codon-EcoRV-localization tag-stop codon-BstBI. New genes can be cloned into the vector with N-term Flag and C-term localization signal using SalI-EcoRV.

growth temp :

Grow in XL10Gold or Stbl3 cells to decrease recombination. Growing the cultures at 30C rather than 37 and in SOC sometimes helps with yield.

origin :

pi :

alt_names

raptor HRas25

cloning

clone_method	Restriction Enzyme
cloning_site_3	BstBI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	KOG1, Mip1
gene	RPTOR
id	57521

genbank_ids

mutation

name

raptor-HRas25

shRNA_sequence

size

species

9606

Homo sapiens