This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLJM1 Flag Raptor
catalog :
26633
citations: 7
Reference
Du X, Di Malta C, Fang Z, Shen T, Niu X, Chen M, et al. Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway. Acta Pharm Sin B. 2022;12:2869-2886 pubmed publisher
Ericksen R, Lim S, McDonnell E, Shuen W, Vadiveloo M, White P, et al. Loss of BCAA Catabolism during Carcinogenesis Enhances mTORC1 Activity and Promotes Tumor Development and Progression. Cell Metab. 2019;: pubmed publisher
Thomas H, Zhang Y, Stefely J, Veiga S, Thomas G, Kozma S, et al. Mitochondrial Complex I Activity Is Required for Maximal Autophagy. Cell Rep. 2018;24:2404-2417.e8 pubmed publisher
Meade N, Furey C, Li H, Verma R, Chai Q, Rollins M, et al. Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit. Cell. 2018;174:1143-1157.e17 pubmed publisher
Su K, Cao J, Tang Z, Dai S, He Y, Sampson S, et al. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nat Cell Biol. 2016;18:527-39 pubmed publisher
Plescher M, Teleman A, Demetriades C. TSC2 mediates hyperosmotic stress-induced inactivation of mTORC1. Sci Rep. 2015;5:13828 pubmed publisher
Sancak Y, Bar Peled L, Zoncu R, Markhard A, Nada S, Sabatini D. Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Cell. 2010;141:290-303 pubmed publisher
product information
Catalog Number :
26633
Product Name :
pLJM1 Flag Raptor
article :
doi10.1016/j.cell.2010.02.024
id3721
pubmed_id20381137
bacterial resistance :
Ampicillin
cloning :
backbonepLJM1
backbone_mutation
backbone_originAvailable at Addgene (#19319)
backbone_size7400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth temp :
Grow in XL10Gold or Stbl3 cells to decrease recombination. Growing the cultures at 30C rather than 37 and in SOC sometimes helps with yield.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BstBI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKOG1, Mip1
geneRPTOR
id57521
genbank_ids
mutation
nameraptor
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
432
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA