pLKO.1 RagC shRNA #2 | Addgene 26630 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLKO.1 RagC shRNA #2

catalog :

26630

citations: 2

Reference
Yang G, Humphrey S, Murashige D, Francis D, Wang Q, Cooke K, et al. RagC phosphorylation autoregulates mTOR complex 1. EMBO J. 2019;38: pubmed publisher
Sancak Y, Peterson T, Shaul Y, Lindquist R, Thoreen C, Bar Peled L, et al. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science. 2008;320:1496-501 pubmed publisher

product information

Catalog Number :

26630

Product Name :

pLKO.1 RagC shRNA #2

article :

doi	10.1126/science.1157535
id	2268
pubmed_id	18497260

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1 puro

backbone_mutation

backbone_origin

Available at Addgene (#8453)

backbone_size

7000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

shRNA directed against RagC. Target sequence: 5'-GTGGATATGCAATCTTATGAA-3'. Sequence from the website of the RNAi consortium at the Broad institute.

origin :

pi :

alt_names

RagC

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	LKO.1 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GTR2, RAGC, TIB929
gene	RRAGC
id	64121

genbank_ids

mutation

name

RagC shRNA

shRNA_sequence

size

species

9606

Homo sapiens