This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-TOP luciferase
catalog :
26611
citations: 2
Reference
Nguyen T, Hector H, Pederson E, Lin J, Ouyang Z, Wendel H, et al. Rapamycin-Induced Feedback Activation of eIF4E-EIF4A Dependent mRNA Translation in Pancreatic Cancer. Cancers (Basel). 2023;15: pubmed publisher
Kim J, Chen J. Cytoplasmic-nuclear shuttling of FKBP12-rapamycin-associated protein is involved in rapamycin-sensitive signaling and translation initiation. Proc Natl Acad Sci U S A. 2000;97:14340-5 pubmed
product information
Catalog Number :
26611
Product Name :
pcDNA3-TOP luciferase
article :
doi10.1073/pnas.011511898
id3715
pubmed_id11114166
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
eEF2 5' UTR was amplified from total RNA.
origin :
37
pi :
alt_names
firefly luciferase
eEF2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5HindIII
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameeEF2 5'UTR
shRNA_sequence
size1732
species
Hamster
tags
plasmid copy :
Luciferase is from pGL2-Basic (Promega).
resistance markers :
818
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA