This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Flag mTOR S2035T
catalog :
26604
citations: 2
Reference
Xu M, Almasi S, Yang Y, Yan C, Sterea A, Rizvi Syeda A, et al. The lysosomal TRPML1 channel regulates triple negative breast cancer development by promoting mTORC1 and purinergic signaling pathways. Cell Calcium. 2019;79:80-88 pubmed publisher
Vilella Bach M, Nuzzi P, Fang Y, Chen J. The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. J Biol Chem. 1999;274:4266-72 pubmed
product information
Catalog Number :
26604
Product Name :
pcDNA3-Flag mTOR S2035T
article :
doi10.1074/jbc.274.7.4266
id3712
pubmed_id9933627
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
NM_004958
mutationS2035T
namemTOR
shRNA_sequence
size7600
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
plasmid copy :
WT mTOR insert is from Stuart Schreiber's lab. Brown et al, Nature 369:756 (1994).
resistance markers :
818
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA