This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Flag mTOR wt
catalog :
26603
citations: 30
Reference
. mTOR Pathway Somatic Pathogenic Variants in Focal Malformations of Cortical Development: Novel Variants, Topographic Mapping, and Clinical Outcomes. Neurol Genet. 2023;9:e200103 pubmed publisher
Li T, Wang Q, Gao A, Li X, Sun Y, Mottis A, et al. Lysosomes mediate the mitochondrial UPR via mTORC1-dependent ATF4 phosphorylation. Cell Discov. 2023;9:92 pubmed publisher
Nicastro R, Broh xe9 e L, Alba J, N xfc chel J, Figlia G, Kipschull S, et al. Malonyl-CoA is a conserved endogenous ATP-competitive mTORC1 inhibitor. Nat Cell Biol. 2023;25:1303-1318 pubmed publisher
Liu H, Du H, Khabibullin D, Zarei M, Wei K, Freeman G, et al. mTORC1 upregulates B7-H3/CD276 to inhibit antitumor T cells and drive tumor immune evasion. Nat Commun. 2023;14:1214 pubmed publisher
Fujita A, Kato M, Sugano H, Iimura Y, Suzuki H, Tohyama J, et al. An integrated genetic analysis of epileptogenic brain malformed lesions. Acta Neuropathol Commun. 2023;11:33 pubmed publisher
Zhang S, Lee S, Nie L, Huang Y, Zou G, Jung Y, et al. Lysosomal TMEM9-LAMTOR4-controlled mTOR signaling integrity is required for mammary tumorigenesis. Cancer Commun (Lond). 2022;: pubmed publisher
Yu Z, Chen J, Takagi E, Wang F, Saha B, Liu X, et al. Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt. J Biol Chem. 2022;298:102288 pubmed publisher
Ganner A, Gehrke C, Klein M, Thegtmeier L, Matulenski T, Wingendorf L, et al. VHL suppresses RAPTOR and inhibits mTORC1 signaling in clear cell renal cell carcinoma. Sci Rep. 2021;11:14827 pubmed publisher
Nüchel J, Tauber M, Nolte J, Morgelin M, Türk C, Eckes B, et al. An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. Mol Cell. 2021;: pubmed publisher
Besterman A, Althoff T, Elfferich P, Gutierrez Mejia I, Sadik J, Bernstein J, et al. Functional and structural analyses of novel Smith-Kingsmore Syndrome-Associated MTOR variants reveal potential new mechanisms and predictors of pathogenicity. PLoS Genet. 2021;17:e1009651 pubmed publisher
Zhang H, Yi J, Huang H, Park S, Park S, Kwon W, et al. Rhein Suppresses Colorectal Cancer Cell Growth by Inhibiting the mTOR Pathway In Vitro and In Vivo. Cancers (Basel). 2021;13: pubmed publisher
Yeh C, Bellon M, Wang F, Zhang H, Fu L, Nicot C. Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells. Mol Cancer. 2020;19:139 pubmed publisher
Kim D, Park G, Huuhtanen J, Lundgren S, Khajuria R, Hurtado A, et al. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease. Nat Commun. 2020;11:2246 pubmed publisher
Xiao B, Zuo D, Hirukawa A, Cardiff R, Lamb R, Sonenberg N, et al. Rheb1-Independent Activation of mTORC1 in Mammary Tumors Occurs through Activating Mutations in mTOR. Cell Rep. 2020;31:107571 pubmed publisher
Lin J, Jiao A, Lv W, Zhang C, Shi Y, Yang Z, et al. Pentapeptide Protects INS-1 Cells From hIAPP-Mediated Apoptosis by Enhancing Autophagy Through mTOR Pathway. Front Pharmacol. 2019;10:896 pubmed publisher
Xu M, Almasi S, Yang Y, Yan C, Sterea A, Rizvi Syeda A, et al. The lysosomal TRPML1 channel regulates triple negative breast cancer development by promoting mTORC1 and purinergic signaling pathways. Cell Calcium. 2019;79:80-88 pubmed publisher
Kovalski J, Bhaduri A, Zehnder A, Neela P, Che Y, Wozniak G, et al. The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Mol Cell. 2019;73:830-844.e12 pubmed publisher
Li X, Zhu G, Yao X, Wang N, Hu R, Kong Q, et al. Celastrol induces ubiquitin-dependent degradation of mTOR in breast cancer cells. Onco Targets Ther. 2018;11:8977-8985 pubmed publisher
Jung S, Hwang H, Kang D, Park H, Lee H, Jeong D, et al. mTOR kinase leads to PTEN-loss-induced cellular senescence by phosphorylating p53. Oncogene. 2019;38:1639-1650 pubmed publisher
Bruning U, Morales Rodriguez F, Kalucka J, Goveia J, Taverna F, Queiroz K, et al. Impairment of Angiogenesis by Fatty Acid Synthase Inhibition Involves mTOR Malonylation. Cell Metab. 2018;28:866-880.e15 pubmed publisher
Gu Y, Albuquerque C, Braas D, Zhang W, Villa G, Bi J, et al. mTORC2 Regulates Amino Acid Metabolism in Cancer by Phosphorylation of the Cystine-Glutamate Antiporter xCT. Mol Cell. 2017;67:128-138.e7 pubmed publisher
Brown M, Gromeier M. MNK Controls mTORC1:Substrate Association through Regulation of TELO2 Binding with mTORC1. Cell Rep. 2017;18:1444-1457 pubmed publisher
Mimoto R, Nihira N, Hirooka S, Takeyama H, Yoshida K. Diminished DYRK2 sensitizes hormone receptor-positive breast cancer to everolimus by the escape from degrading mTOR. Cancer Lett. 2017;384:27-38 pubmed publisher
Chen Y, Xu J, Skanderup A, Dong Y, Brannon A, Wang L, et al. Molecular analysis of aggressive renal cell carcinoma with unclassified histology reveals distinct subsets. Nat Commun. 2016;7:13131 pubmed publisher
Su K, Cao J, Tang Z, Dai S, He Y, Sampson S, et al. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nat Cell Biol. 2016;18:527-39 pubmed publisher
Bhardwaj A, Ganesan N, Tachibana K, Rajapakshe K, Albarracin C, Gunaratne P, et al. Annexin A1 Preferentially Predicts Poor Prognosis of Basal-Like Breast Cancer Patients by Activating mTOR-S6 Signaling. PLoS ONE. 2015;10:e0127678 pubmed publisher
Li C, Chen J, Lu B, Shi Z, Wang H, Zhang B, et al. Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and autophagy in human non-small cell lung cancer A549 cells. PLoS ONE. 2014;9:e101526 pubmed publisher
Jha A, Ahuja M, Patel S, Brailoiu E, Muallem S. Convergent regulation of the lysosomal two-pore channel-2 by Mg²?, NAADP, PI(3,5)P? and multiple protein kinases. EMBO J. 2014;33:501-11 pubmed publisher
Wu X, Bhayani M, Dodge C, Nicoloso M, Chen Y, Yan X, et al. Coordinated targeting of the EGFR signaling axis by microRNA-27a*. Oncotarget. 2013;4:1388-98 pubmed
Vilella Bach M, Nuzzi P, Fang Y, Chen J. The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. J Biol Chem. 1999;274:4266-72 pubmed
product information
Catalog Number :
26603
Product Name :
pcDNA3-Flag mTOR wt
article :
doi10.1074/jbc.274.7.4266
id3712
pubmed_id9933627
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression of human flag-tagged mTor
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
NM_004958
mutation
namemTOR
shRNA_sequence
size7650
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
plasmid copy :
WT mTOR insert is from Stuart Schreiber's lab. Brown et al, Nature 369:756 (1994).
resistance markers :
818
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA