CaV1.3e[8a,11,31b, 32, 42a] mut | Addgene 26577 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CaV1.3e[8a,11,31b, 32, 42a] mut

catalog :

26577

citations: 3

Reference
Abderemane Ali F, Findeisen F, Rossen N, Minor D. A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel Calcium-Dependent Inactivation. Neuron. 2019;101:1134-1149.e3 pubmed publisher
Moreno C, Dixon R, Tajada S, Yuan C, Opitz Araya X, Binder M, et al. Ca(2+) entry into neurons is facilitated by cooperative gating of clustered CaV1.3 channels. elife. 2016;5: pubmed publisher
Xu W, Lipscombe D. Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines. J Neurosci. 2001;21:5944-51 pubmed

product information

Catalog Number :

26577

Product Name :

CaV1.3e[8a,11,31b, 32, 42a] mut

article :

doi
id	3699
pubmed_id	11487617

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA6/V5-His ABC

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Isolated from superior cervical ganglia. For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm

growth temp :

Top10F'

inserts :

Top10F'

origin :

pi :

alt_names

calcium channel, voltage-dependent, L type, alpha 1D subunit

Cav1.3

cloning

clone_method	Restriction Enzyme
cloning_site_3	SacI
cloning_site_5	NheI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Cacna1d
id	29716

genbank_ids

mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a are deleted. Exon 42a was replaced by exons 42 & 49. nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731(TCA->GGA) [Ser->Gly].

name

CACNA1D

shRNA_sequence

size

6400

species

10116

Rattus norvegicus