This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CaV1.3e[8a,11,31b, 32,42a] mut
catalog :
26576
citations: 4
Reference
Yang T, He M, Zhang H, Barrett P, Hu C. L- and T-type calcium channels control aldosterone production from human adrenals. J Endocrinol. 2020;244:237-247 pubmed publisher
Hristov K, Mangalanathan U, Casciola M, Pakhomova O, Pakhomov A. Expression of voltage-gated calcium channels augments cell susceptibility to membrane disruption by nanosecond pulsed electric field. Biochim Biophys Acta Biomembr. 2018;1860:2175-2183 pubmed publisher
Moreno C, Dixon R, Tajada S, Yuan C, Opitz Araya X, Binder M, et al. Ca(2+) entry into neurons is facilitated by cooperative gating of clustered CaV1.3 channels. elife. 2016;5: pubmed publisher
Xu W, Lipscombe D. Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines. J Neurosci. 2001;21:5944-51 pubmed
product information
Catalog Number :
26576
Product Name :
CaV1.3e[8a,11,31b, 32,42a] mut
article :
doi
id3699
pubmed_id11487617
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA6/V5-His ABC
backbone_mutation
backbone_originInvitrogen
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Isolated from superior cervical ganglia. For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm
growth temp :
Top10F'
inserts :
Top10F'
origin :
37
pi :
alt_names
calcium channel, voltage-dependent, L type, alpha 1D subunit
Cav1.3
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCacna1d
id29716
genbank_ids
mutationContains exons 8a, 11, 31b and 42a.Deletion of exon 32. nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
nameCACNA1D
shRNA_sequence
size5700
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagV5
locationC terminal on backbone
tag6XHis
resistance markers :
813
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA