This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MG-155
catalog :
26529
citations: 3
Reference
Jost M, Chen Y, Gilbert L, Horlbeck M, Krenning L, Menchon G, et al. Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent. Mol Cell. 2017;68:210-223.e6 pubmed publisher
Gaudette B, Dwivedi B, Chitta K, Poulain S, Powell D, Vertino P, et al. Low expression of pro-apoptotic Bcl-2 family proteins sets the apoptotic threshold in Waldenström macroglobulinemia. Oncogene. 2016;35:479-90 pubmed publisher
O Connell R, Rao D, Chaudhuri A, Boldin M, Taganov K, Nicoll J, et al. Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder. J Exp Med. 2008;205:585-94 pubmed publisher
product information
Catalog Number :
26529
Product Name :
MG-155
article :
doi10.1084/jem.20072108
id3686
pubmed_id18299402
bacterial resistance :
Ampicillin
cloning :
backbonepMIG-w
backbone_mutation
backbone_originAddgene plasmid 12282
backbone_size6500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
MG155 was derived originally from pMIG-w. The GFP and IRES were removed using BglII and HindIII, and a new cassette with eGFP (about 700 bps) followed by a miR-155 sequence (about 500 bps with flanking Not1 and XhoI sites) was cloned in using BamHI (destroying the BglII site) and HindIII. This results in a 7 kb plasmid.
origin :
37
pi :
alt_names
BIC (part of Exon3)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoter
sequencing_primer_3MSCV-rev
sequencing_primer_5pLXSN-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN155, miRNA155, mir-155
geneMIR155
id406947
genbank_ids
mutation
namemiR-155
shRNA_sequence
size500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tageGFP
resistance markers :
20
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA